A rapid qualitative and quantitative detection kit for oil adjuvant vaccine and its detection method

Abstract

The invention provides a fast qualitative and quantitative detection kit of an oil adjuvant vaccine, and a detection method of the fast qualitative and quantitative detection kit. The detection kit comprises the following components: a demulsifier, an injector and a colourimetric card; the kit also comprises rapid test strips or a 96-well plate; the demulsifier is one of an ethanol solution containing 0.33% by volume of hydrochloric acid, and an acetonitrile solution containing 0.05% by volume of trifluoroacetic acid. The kit provided by the invention can be used for qualitatively and quantitatively detecting the content of protein in the oil adjuvant vaccine rapidly; the kit is easy to manufacture and simple and convenient to operate, no complicated pretreatment is needed, and detection results are accurate, so that a fast and effective detection means is provided for the industry of foot-and-mouth disease vaccines.

Description

technical field [0001] The invention relates to the technical field of foot-and-mouth disease vaccine detection, in particular to a rapid qualitative and quantitative detection kit for oil adjuvant vaccines and a detection method thereof. Background technique [0002] The existing foot-and-mouth disease vaccines on the market are mainly divided into inactivated vaccines and synthetic peptide vaccines. The core of the antigen is protein. The detection of the antigen in the vaccine is mainly to detect the protein content in the water phase of the vaccine after demulsification. However, the foot-and-mouth disease vaccine is emulsified by antigen and adjuvant in a certain ratio and according to a specific procedure. Since the antigen is wrapped in oil adjuvant, it cannot be directly detected. Although there are demulsification methods in the industry, their efficiency and effect are not good. There will be various problems after demulsification, such as incomplete demulsificatio...

AI Technical Summary

Problems solved by technology

However, the foot-and-mouth disease vaccine is emulsified by antigen and adjuvant in a certain ratio and according to a specific procedure. Since the antigen is wrapped in oil adjuvant, it cannot be directly detected

Although there are demulsification methods in the industry, their efficiency and effect are not good. There will be various problems after demulsification, such as incomplete demulsification, unclear boundary between water phase and oil phase, and no antigen detected in the water phase. plagued the industry

At present, the national inspection of vaccines is gradually transitioning to the detection of antigens in vaccines, and the current more common method is to detect antigens after vaccine demulsification. By demulsifying the vaccine, the antigens are transferred to the water phase, and then For follow-up detection and analysis, although there are many protein detection methods, problems such as demulsification and adjuvant interference cannot be solved. Therefore, there is no uniform and accurate method for the inspection of foot-and-mouth disease vaccines in the industry. For vaccine users There is even a lack of rapid qualitative and quantitative detection methods

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