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Cremastra appendiculata seedling method

A technology of rhododendron and rhododendron pods, applied in the biological field, can solve the problems of long time period, cumbersome process, high cost, etc., and achieve the effect of saving manpower and financial resources

Active Publication Date: 2017-06-09
CHENGDU URBAN MODERN AGRI IND TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Later, scientific research workers developed the method of cultivating rhododendron orchid seedlings under sterile tissue culture conditions. The disadvantages are that the process is cumbersome, the time period is long, and the cost is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A, seed selection: select Rhododendron orchid pod seeds, and carry out disinfection process to Rhododendron orchid pod seeds in aseptic environment;

[0039] B, preparation medium: described medium is made up of 1 / 2MS, 4g / L agar, 0.01mg / LNAA, and the pH value of described medium is 5.8, and the prepared medium is packed in the tissue culture bottle;

[0040] C, inoculation: the sterilized Rhododendron pod seeds are evenly sprinkled on the culture medium in the tissue culture bottle;

[0041] D. Cultivation in the culture room: Place the inoculated tissue culture bottle in a sterile environment with a light intensity of 2000lx and a temperature of 25°C for 45 days, and the light time is 10h / d;

[0042] E. Seedling hardening: After the cultivation in the cultivation room, observe that the seedlings grow two roots of about 1 cm, and when they have two leaves, place them in an outdoor greenhouse without direct sunlight for 7 days at a temperature of 20-30°C;

[0043] F, ou...

Embodiment 2

[0048] A, seed selection: select Rhododendron orchid pod seeds, and carry out disinfection process to Rhododendron orchid pod seeds in aseptic environment;

[0049] B, preparation medium: described medium is made up of MS, 4g / L agar, 0mg / LNAA, and the pH value of described medium is 5.8, and the prepared medium is packed in tissue culture bottle;

[0050] C, inoculation: the sterilized Rhododendron pod seeds are evenly sprinkled on the culture medium in the tissue culture bottle;

[0051] D. Cultivation in the culture room: Place the inoculated tissue culture bottle in a sterile environment with a light intensity of 2000lx and a temperature of 27°C for 45 days, and the light time is 12h / d;

[0052] E. Seedling hardening: After the cultivation in the cultivation room, observe that the seedlings grow two roots of about 1 cm, and when they have two leaves, place them in an outdoor greenhouse without direct sunlight for 4 days at a temperature of 20-30°C;

[0053] F, out-of-the-b...

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Abstract

The invention discloses a cremastra appendiculata seedling method. The method includes seed selection, culture medium preparation, inoculation, culture-room cultivation, seedling hardening, bottle-out cultivation, fertilization and transplantation. Group-culture seedling and seedling hardening method and process are improved, a culture medium formula that group-culture seedlings start to take root in the first stage, the following nearly three-month group-culture time is omitted, the transfer process of twice group-culture seedlings is omitted, a great deal of man power and financial resources are saved, sufficient time is provided for production of the next batch of cremastra appendiculata seedlings, and the cremastra appendiculata seedling method is suitable for promotion and application in the technical biotechnilogies.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for growing seedlings of rhododendron orchids. Background technique [0002] Rhododendron orchid is a perennial herbaceous plant of the genus Rhododendron orchid in the family Orchidaceae. Its pseudobulb is called Shanzi mushroom or Maozi mushroom after drying, which can be used as medicine and is an important and scarce Chinese medicinal material. Modern medical research shows that the internal use of Sanzi mushroom has anti-tumor effect, and the external use can treat sores, insect bites, skin scalds or burns, etc. Due to people's long-term predatory mining and the limitation of their own reproductive mechanism, resources are exhausted. With the increasing demand for rhododendron orchids in the medicinal material market, the rapid propagation of rhododendron orchids has become a difficult problem to be solved urgently. Later, scientific research workers deve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄琳凯曹欣文兴金张新全陈佩琳武炳超王成然陈静
Owner CHENGDU URBAN MODERN AGRI IND TECH RES INST CO LTD
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