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Method for detecting tobacco leaf reducing sugar

A detection method and technology of tobacco leaves, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of simple extraction method, simple measurement method, unstable detection results, etc., and achieve the effect of accurate detection and good repeatability

Inactive Publication Date: 2017-06-09
驻马店市烟草公司泌阳县分公司
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Problems solved by technology

Fehling's reagent colorimetric method and 3,5-dinitrosalicylic acid colorimetric method (DNS) are the most commonly used methods at present, but both have some shortcomings. The former extraction method is simple and the determination process is cumbersome; the latter determination The method is simple but the extraction takes a long time and the detection results are unstable

Method used

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with embodiment:

[0019] The steps of the present invention are as follows:

[0020] a. Tobacco leaf reducing sugar extraction:

[0021] Weigh 100 mg of tobacco leaves, put them into a 20 mL centrifuge tube, add 10 mL of boiling water, boil for 5 min, add water to 15 mL, shake fully and filter through filter paper to obtain reducing sugar extract for detection.

[0022] b. Reaction with picric acid and sodium carbonate:

[0023] Take 0.5 mL of the extract to be tested, add 0.3 mL of picric acid with a concentration of 0.5%, and 1.5 mL of sodium carbonate with a concentration of 20% in sequence, heat it in boiling water for 10 min, and then cool it in cold water for 2 min.

[0024] c. Detection:

[0025] The absorbance of the reaction solution was detected using a wavelength of 285 nm.

[0026] d, compared with the standard curve data to determine the reducing sugar content:

[0027] Compare the d...

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Abstract

The invention discloses a method for detecting tobacco leaf reducing sugar. The method comprises the following steps: manufacturing a reducing sugar standard curve: adding a certain quantity of picric acid into glucose solutions with different concentrations, performing a heating reaction on sodium carbonate in boiled water for 10 min, detecting the light absorption values at 285 nm, and manufacturing the standard curve according to the concentrations and the light absorption values; and extracting soluble reducing sugars in tobacco leaves with boiled water, then adding a certain quantity of picric acid, performing a heating reaction on sodium carbonate in boiled water for 10 min to obtain a tobacco leaf reducing sugar to-be-detected solution, detecting the light absorption values at 285 nm, and calculating the content of the tobacco leaf reducing sugar according to the standard curve. The method has the advantages that the detection result is reliable, the repeatability is high, the operation is simple and convenient, popularization is convenient, and the method can be not only used for detecting the tobacco leaf reducing sugar but also widely applied to preliminary determination of other soluble reducing sugar.

Description

technical field [0001] The invention belongs to the technical field of tobacco leaf reducing sugar detection, and in particular relates to a method for measuring reducing sugar content. Background technique [0002] Tobacco occupies an extremely important position in China's economic and social development. The reducing sugar content is one of the important indicators for identifying the quality of flue-cured tobacco. The reducing sugar content of tobacco leaves is an indicator that must be detected in tobacco leaf testing technology. [0003] The classic determination method of reducing sugar in flue-cured tobacco leaves is copper reduction-titration method, such as Munson-Walker method and Bo Chuan method. Molybdic acid colorimetric method, o-toluidine colorimetric method, sulfuric acid-anthrone method, etc. Fehling's reagent colorimetric method and 3,5-dinitrosalicylic acid colorimetric method (DNS) are the most commonly used methods at present, but both have some shortc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 吴俊林王迅贾彦军李翔毕乐乐王璀璨吕浩秋何平赵佳佳郝浩浩贾峰
Owner 驻马店市烟草公司泌阳县分公司
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