A preparation method of bovine serum albumin-fenoxycarb nano-medicine for preventing and treating groove-orbital phenomenon

Abstract

The invention discloses a preparation method of bovine serum albumin-coated fenoxycarb nano-medicine for preventing and treating groove-orbital phenomenon. The process of wrapping fenoxycarb with bovine serum albumin for preventing and treating groove-orbital phenomenon adopts a physical embedding method. First, take a certain amount of bovine serum albumin (BSA) and dissolve it in double-distilled water to form an aqueous phase, and take a certain amount of fenoxycarb and dissolve it in acetonitrile to form an oil phase. Then, under the condition of high-speed stirring, the oil phase is added dropwise into the water phase, after emulsification for a period of time, it is cooled rapidly, ultrasonically pulverized to obtain a microemulsion, and cooled. Finally, freeze-dry to obtain fenoxycarb albumin nanoparticles. The new albumin nanoparticle preparation technology adopted in the method is to wrap the drug in the albumin without changing the albumin structure, which increases the drug loading and also has the effect of sustained release. The use of nanotechnology can reduce the amount, residue, cost and environmental pollution of chemical pesticides. At the same time, it can also improve the efficacy of pesticides, effectively increase the contact area between pesticides and target pests, and truly realize the high efficiency, low toxicity and safety of chemical pesticides.

Description

technical field [0001] The invention discloses a preparation method of bovine serum albumin-fenoxycarb nano-medicine for preventing and treating groove-orbital phenomenon, and the preparation process of fenoxycarb nano-particles belongs to the technical fields of life science, chemistry, nano-materials and new pesticides. Background technique [0002] Fenoxycarb, English name Ethyl 2-(4-phenoxyphenoxy)ethylcarbamate , the molecular formula is C 17 h 19 NO 4 , fenoxycarb, fenoxycarb or fenoxycarb, benzene is a non-terpene insect growth regulator insecticide with juvenile hormone activity, which was first put on the market by the Swiss company Syngenta in the 1990s , and was listed by Syngenta as one of the dozen or so reserved varieties in the 21st century. The pure product of fenoxycarb is a colorless crystal, melting point 53-54 ℃, solubility (at 25 ℃): 5.7mg / Kg in water, more than 250g in acetone, chloroform, ethyl ether acetate, methanol, isopropanol, toluene, etc. / ...

AI Technical Summary

Problems solved by technology

Use the embedding technology to make pure drug nanoparticles into nano-biological pesticide microspheres, and play the unique volume effect, surface effect, quantum size effect and macro-quantum tunnel effect of nanomaterials, and significantly improve the efficacy of pesticides. Traditional pesticide application methods It is through the spraying method, through which 80-90% of the active ingredients of the drug will be lost to the soil and the air, directly resulting in an increase in the amount of pesticide application and the increase in the frequency of application, etc.

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