Hard-tissue cutter for primary cell culture

Abstract

The invention relates to a hard-tissue cutter for primary cell culture. The hard-tissue cutter comprises a collecting pipe, a spring device and a cutting device, wherein the spring device comprises an upper disc, a spring and a lower disc; one end of the spring is connected with the upper disc, and the other end of the spring is connected with the lower disc; the other end of the lower disc is also provided with a joint; the cutting device also comprises a pressing rod and cutting heads; the cutting heads comprise cutting head bodies, ring-shaped blades and U-shaped blades; and the cutting heads are divided into first cutting heads and second cutting heads according to the spacing distance. The hard-tissue cutter has the advantages that the principle of the device is piston motion, the structure is simple, and the operation is convenient, so that the time for cutting up tissues is greatly shortened, further the exposure time of the tissues in the air is reduced, unnecessary pollution is avoided, and the probability of successful culture of primary cells is increased.

Description

technical field [0001] The invention relates to the technical field of primary cell culture equipment, in particular to a hard tissue cutter for primary cell culture. Background technique [0002] Primary cell culture means that various tissues of the animal body are taken out from the body, treated with various enzymes (usually trypsin), chelating agents (usually EDTA) or mechanical methods, dispersed into single cells, and placed in a suitable medium Medium culture, a biotechnology that enables cells to survive, grow and reproduce. [0003] Primary cell culture, also known as primary cell culture, refers to culturing cells, tissues and organs directly from the body. Primary cell culture technology is a powerful means to study the growth, metabolism and reproduction of living cells, and it also creates favorable conditions for subsequent subculture. Because the cells have just been separated from the living tissue, they are closer to the living conditions in the organism,...

AI Technical Summary

Problems solved by technology

[0008] 1. Due to structural limitations, it is not possible to operate in the ultra-clean bench, and if the hard tissue used for primary cell culture is cut outside the ultra-clean bench, it will cause tissue contamination, which will lead to the failure of the experiment;

[0009] 2. The cutting direction is single. In the patent literature, the cutting is achieved by setting the distance between the double saw blades. However, the hard tissue used for primary cell culture needs to be cut into uniform tissue blocks from different directions to meet the requirements.

[0011] 4. Cutting is time-consuming and labor-intensive, and the efficiency is low

However, there is no report about this hard tissue cutter

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