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Mixed microbial agent for degrading proteins and fats in sewage, and preparation method and application thereof

A technology for mixing bacterial agents and degrading sewage, applied in the field of engineering bacteria, can solve the problems of blackening and odor in water body and reducing dissolved oxygen content, and achieve the effects of enhancing metabolism, enriching resources, and expanding a broad spectrum of functions.

Inactive Publication Date: 2017-06-16
SHANGQIU NORMAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Once the wastewater containing these substances enters the water, the dissolved oxygen content will decrease, which will cause the water to turn black and smelly

Method used

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  • Mixed microbial agent for degrading proteins and fats in sewage, and preparation method and application thereof
  • Mixed microbial agent for degrading proteins and fats in sewage, and preparation method and application thereof
  • Mixed microbial agent for degrading proteins and fats in sewage, and preparation method and application thereof

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preparation example Construction

[0026] The preparation method of the mixed bacterial agent for degrading protein and fat in sewage, the steps are as follows:

[0027] a. Activation of strains: On the activation medium, inoculate preserved Candida lipolytica, Candida parapsilosis, Bacillus amylolytica and Pseudomonas putida strains respectively, and culture them at 25°C for 2-3 days. Activate the strain; inoculate the psychrophilic Bacillus on the activation medium and culture it at 15°C for 2-3 days to activate the strain;

[0028] b. Enrichment culture of strains: inoculate the activated Candida lipolytica, Candida parapsilosis, Bacillus amylolytica and Pseudomonas putida on the enrichment medium respectively, at 25-30°C, Shake culture under the condition of 150r / min for 24 hours, inoculate the activated psychrophilic bacillus on the enrichment medium, shake and culture at 10-20°C for 24 hours under the condition of 150r / min, when the OD of the five bacterial solutions When the value reaches 0.5-0.8, and t...

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Abstract

The invention belongs to the field of engineering bacteria, relates to a sewage treatment method, and concretely relates to a mixed microbial agent for degrading proteins and fats in sewage, and a preparation method and an application thereof. The mixed microbial agent contains Bacillus psychrophilus, Candida lipolytica, Candida parapsilosis, Bacillus amyloliquefaciens and Pseudomonas putida, wherein a living microbial quantity ratio of the Bacillus amyloliquefaciens to the Bacillus psychrophilus to the Pseudomonas putida to the Candida lipolytica to the Candida parapsilosis of 2:2:2:(2-4.5):(2-4.5). The preparation method of the mixed microbial agent includes the following steps: activation of strains, enrichment culture of the strains, simultaneous growth of the strains, and mixing of the strains. The mixed microbial agent can be used to degrade the proteins and the fats in the sewage in order to purify water.

Description

technical field [0001] The invention belongs to the field of engineering bacteria and relates to a sewage treatment method, in particular to a mixed bacterial agent for degrading protein and fat in sewage and a preparation method and application thereof. Background technique [0002] With the growth of population, the rapid improvement of urbanization level and the transformation of people's life and production methods, the amount of domestic sewage continues to increase, but the public environmental protection facilities are seriously lagging behind, causing a large amount of domestic sewage to be directly discharged into urban rivers without treatment, causing serious pollution The river water body has been destroyed, causing the organic pollution of the river water to continue to aggravate. The organic matter in urban domestic sewage mainly includes humic acid, protein, lipid, sugar, amino acid, etc. These substances exist in the wastewater in a suspended or dissolved sta...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/07C12R1/73C12R1/72C12R1/40C02F101/34C02F101/38
CPCC02F3/341C02F3/343C02F3/347C02F2101/34C02F2101/38C12N1/16C12N1/20
Inventor 杨广礼李治国院玲玲杜文涛张淑红
Owner SHANGQIU NORMAL UNIVERSITY
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