SSR marker primer group for identifying robinia pseudoacacia germplasm resource genetic relationship and application of SSR marker primer group

A technology for labeling primers and kinship, which is applied in the biological field to reduce the cost and time of experiments, ensure accuracy, and shorten the identification time.

Active Publication Date: 2017-06-20
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since there are no related reports on the use of SSR markers to identify the genetic relationship of Robinia pseudoacacia germplasm resources, how to use relevant SSR markers for detection, thereby improving the accuracy and reliability of detection has become the key to solving the above problems

Method used

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  • SSR marker primer group for identifying robinia pseudoacacia germplasm resource genetic relationship and application of SSR marker primer group
  • SSR marker primer group for identifying robinia pseudoacacia germplasm resource genetic relationship and application of SSR marker primer group
  • SSR marker primer group for identifying robinia pseudoacacia germplasm resource genetic relationship and application of SSR marker primer group

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Referring to the 21 pairs of SSR primers of Robinia pseudoacacia developed by Lian C et al. (2002, 2004) and Mishima K et al. (2009), 16 pairs of primers with high polymorphism and good versatility were selected.

[0060] The 16 pairs of primers used are shown in Table 1:

[0061] Table 1 SSR primers used for the identification of Robinia pseudoacacia gene strains, small clones and population genetic structure analysis

[0062]

[0063]

Embodiment 2

[0065] Sample source

[0066] Taking the pure black locust forest on the west slope of the small dam in the garden of Mount Tai as the research object (east longitude: 117°3′35.96″; north latitude: 36°16′56.26″; altitude: 654.9m). Afforestation was carried out in 1958, inter-cutting in 1976, clear-cutting in 1980, Huashan pine was planted in 1984 with fixed plants and terraced fields, and Huashan pine was removed in 1986 and developed into a sprout forest.

[0067] sampling process

[0068] Select a range of 20m × 15m in the sample plot, and conduct SSR marker analysis on 29 Robinia pseudoacacia individuals;

[0069] Select a 20m×15m sample plot in a pure Robinia pseudoacacia forest, take 29 Robinia pseudoacacia individuals in the sample plot as the research object, take the leaves or branches of 29 Robinia pseudoacacia individuals, put them into kraft paper bags filled with silica gel, and mark the sample number.

[0070] A method for identifying genetic relationship of Rob...

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PUM

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Abstract

The invention relates to a SSR marker primer group for identifying robinia pseudoacacia germplasm resource genetic relationship and application thereof. The SSR marker primer group for identifying robinia pseudoacacia germplasm resource genetic relationship comprises four groups, wherein each group comprises four pairs of primers. The invention also relates to a method for identifying the robinia pseudoacacia germplasm resource genetic relationship by using the primer group. The SSR marker primer group is used for identifying the robinia pseudoacacia germplasm resource genetic relationship for the first time; 16 SSR markers are screened, and the specific primers are designed, so that the identification accuracy is ensured; the identification time can be obviously shortened through being compared with that of the existing identification method.

Description

technical field [0001] The invention relates to an SSR marker primer set for identifying the genetic relationship of Robinia pseudoacacia germplasm resources and an application thereof, belonging to the technical field of biotechnology. Background technique [0002] SSR, referred to as Simple sequence repeats (SSR), is a molecular marker based on PCR (gene amplification) technology, which has the advantages of high polymorphism, repeatability, co-dominance, richness, etc. The study of genetic diversity is of great significance. The genetic relationship is analyzed by determining the genetic distance. When the genetic distance is zero, it indicates that the plants come from the same clone. [0003] Chinese patent document CN102286613A (application number 201110127982.7) discloses a breeding method for preventing the degradation of Gastrodia elata, which uses AFLP or ISSR and SSR and gastrodin content markers to simultaneously detect Gastrodia elata, and draws a clustering dia...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/156C12Q2525/151C12Q2563/107
Inventor 李传荣王灿灿王年申卫星郭慧玲张兴忠
Owner SHANDONG AGRICULTURAL UNIVERSITY
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