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Method for quickly detecting Staphylococcus aureus

A staphylococcus and detection method technology, applied in the detection field of Staphylococcus aureus, can solve the problems of long detection cycle (generally takes 4-7 days, cannot meet the rapid detection of pathogenic bacteria, heavy preparation and finishing work, etc., and achieve saving Clean water, electricity and storage, shorten labor time, and facilitate on-site inspection

Inactive Publication Date: 2017-06-27
河南艾能生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of Staphylococcus aureus mostly adopts the traditional microbial culture method, which mainly includes the steps of pre-enrichment, selective enrichment, plate separation, biochemical test and serological identification, etc., which involves many experiments and cumbersome operations. The cycle is long (generally takes 4-7 days), the preparation and finishing work are heavy, and it can no longer meet the actual needs of rapid detection of pathogenic bacteria in food at this stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A rapid detection method for Staphylococcus aureus, comprising the following steps:

[0017] 1. Culture medium preparation

[0018] 1) Preparation of enrichment medium: Weigh 26.5 g of enrichment medium, add 1 L of preheated 42 ℃ deionized water, fully dissolve, and set aside;

[0019] 2) Preparation of selective medium: weigh 7.5 g of selective medium, add 100 mL of preheated 42°C deionized water, fully dissolve, and set aside;

[0020] 2. Aseptic operation Take 25 g (mL) samples into a homogenization bag, add 225 mL of preheated enrichment medium to it, put it in a homogenizer for 2 min; culture at 42 °C statically or with shaking 6 h, it can be extended to 24 h as needed; take 0.1 mL of the above enrichment solution, add it into a 5 mL glass tube containing 1 mL of selective medium, and continue to incubate at 42 °C for 21 h;

[0021] 3. Mix the culture evenly, take 1 mL into a test tube, heat it with boiling water (100 ℃) for 10 min, cool to room temperature, add ...

Embodiment 2

[0025] A rapid detection method for Staphylococcus aureus, comprising the following steps:

[0026] 1. Culture medium preparation

[0027] 1) Preparation of enrichment medium: Weigh 26.5 g of enrichment medium, add 1 L of preheated 43 ℃ deionized water, fully dissolve, and set aside;

[0028] 2) Preparation of selective medium: Weigh 7.5 g of selective medium, add 100 mL of preheated 43 ℃ deionized water, fully dissolve, and set aside;

[0029] 2. Aseptic operation Take 25 g (mL) samples into a homogenization bag, add 225 mL of preheated enrichment medium to it, put it in a homogenizer for 2 min; culture at 42 °C statically or with shaking 6 h, and it can be extended to 24 h as needed; take 0.1 mL of the above enrichment solution, add it into a 5 mL glass tube containing 1 mL of selective medium, and continue to incubate at 43 °C for 18-24 h;

[0030] 3. Mix the culture evenly, take 1 mL into a test tube, heat it with boiling water (100 ℃) for 10 min, cool to room temperatur...

Embodiment 3

[0034] A rapid detection method for Staphylococcus aureus, comprising the following steps:

[0035] 1. Culture medium preparation

[0036] 1) Preparation of enrichment medium: Weigh 26.5 g of enrichment medium, add 1 L of preheated 41°C deionized water, fully dissolve, and set aside;

[0037] 2) Preparation of selective medium: Weigh 7.5 g of selective medium, add 100 mL of preheated 41 ℃ deionized water, fully dissolve, and set aside;

[0038] 2. Aseptic operation Take 25 g (mL) samples into a homogenization bag, add 225 mL of preheated enrichment medium to it, put it in a homogenizer for 2 min; culture at 42 °C statically or with shaking 6 h, it can be extended to 24 h as needed; take 0.1 mL of the above enrichment solution, add it into a 5 mL glass tube containing 1 mL of selective medium, and continue to incubate at 41 °C for 18-24 h;

[0039] 3. Mix the culture evenly, take 1 mL into a test tube, heat it with boiling water (100 ℃) for 10 min, cool to room temperature, a...

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Abstract

The invention provides a method for quickly detecting Staphylococcus aureus and relates to the technical field of detection of Staphylococcus aureus. The method comprises the steps of 1, preparing a culture medium; 2, loading 25 g (mL) of a sample into a homogenizing bag in aseptic manner, adding 225 mL of an enrichment medium preheated, and homogenizing in a homogenizer for 2 min; standing at 42 DEG C or culturing by shaking for 6 hours, optionally 24 hours as required; adding 0.1 mL of the enriched liquid into a 5 mL glass tube containing 1 mL of a selective medium, continuing to culture at 42+ / -1 DEG C for 18-24 hours; 3, mixing well the culture, adding 1 mL of the mixture into boiling water (100 DEG C) in the test tube, heating for 10 min, cooling to room temperature, dropwise adding 3-4 drops to a sample hole of a colloidal gold detection card through a dropper, allowing reaction for 5-10 min, judging results, and determining failure if developing takes longer than 30 min. The method is a convenient effective means to quickly and qualitatively detect Staphylococcus aureus.

Description

technical field [0001] The invention relates to the technical field of detection of Staphylococcus aureus, in particular to a rapid detection method of Staphylococcus aureus. Background technique [0002] Staphylococcus aureus is an important pathogenic bacterium of humans, belonging to the genus Staphylococcus (Staphylococcus). Staphylococcus aureus is ubiquitous in nature and can be found in air, water, dust, and human and animal waste. Therefore, there are many opportunities for food to be contaminated. The U.S. Centers for Disease Control reports that Staphylococcus aureus is the second-leading infection after E. coli. Staphylococcus aureus enterotoxin is a worldwide health problem. In the United States, food poisoning caused by Staphylococcus aureus enterotoxin accounts for 33% of the total bacterial food poisoning, and Canada has more, accounting for 45%. Food poisoning incidents caused by cocci also occur from time to time. [0003] Accurate and timely detection m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
CPCG01N33/531G01N33/56938G01N2333/31
Inventor 李东亮黄二辉张广雷
Owner 河南艾能生物科技有限公司
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