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Method for promoting Luteolin accumulation in honeysuckle bud by using fungi

A technology of luteolin and fungi is applied in the field of promoting the accumulation of secondary metabolites in honeysuckle flower buds, which can solve the problems of few phyllostachys in the cotyledon, and no reports of uses, and achieves convenient operation, wide source of raw materials, and improved quality. Effect

Active Publication Date: 2017-07-04
山东中平药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the fungus phyllophyllum, mainly to make it into food or medicinal raw materials, and other uses have not been reported.

Method used

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  • Method for promoting Luteolin accumulation in honeysuckle bud by using fungi
  • Method for promoting Luteolin accumulation in honeysuckle bud by using fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Adopt the following methods to cultivate honeysuckle and take flower buds:

[0031] 1. Preparation of honeysuckle cuttings

[0032]In early April, choose a cloudy day at around 9:00 am to collect cuttings, select two-year-old twigs or hard branches with a thickness of 4 mm, strong growth, no pests, no shrinkage, and no skin damage, and cut them to grow to about 30 cm. For cuttings, there are 6-8 nodes on each cutting, and 1 / 2 leaves are cut off from the leaves of each node. Tie the cut branches into 1 bundle every 50, and spray the branches with a sprayer. After the branches are all wet, put them into a foam heat insulation box, cover with a layer of wet towel to moisturize, cover the lid, and wait. cuttings.

[0033] , Preparation of fungal liquid

[0034] 2.1 Preparation of tea currant phyllodes fungus liquid

[0035] A. Preparation of Media

[0036] Preparation of slant medium: buy potatoes in the market, peel off the outer skin, cut into small pieces of 1 cm sq...

Embodiment 2

[0057] According to the method of Example 1, honeysuckle was cultivated, and flower buds were collected, the difference being that the volume ratio of the fungus liquid of the leafy layer of the currant seedlings and the liquid of Aspergillus oryzae was 2:1.

Embodiment 3

[0059] According to the method of Example 1, honeysuckle was cultivated, and the flower buds were collected, except that the volume ratio of the fungus liquid in the leaf layer of the currant seedlings and the liquid of Aspergillus oryzae was 8:1.

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Abstract

The invention discloses a method for promoting Luteolin accumulation in honeysuckle bud by using fungi, and the method the comprises the steps of cultivating a honeysuckle by cutting seedling, and inoculating fungi to the honeysuckle branch in the cutting process to improve Luteolin content in the honeysuckle bud. The fungi are cynodon dactylon and Aspergillus oryzae. The fungi is used to process the honeysuckle plant, making the honeysuckle grow in the accompany of the fungi's growth; the fungi grows in the stem base of the honeysuckle; the growth of the fungi can promote the accumulation of secondary metabolites in the honeysuckle bud, thereby raising honeysuckle flower quality. The raw material source is wide; the operation is convenient; the method can be easily carried out; and the method has an important meaning in raising honeysuckle flower quality.

Description

technical field [0001] The invention relates to a method for promoting the accumulation of secondary metabolites in honeysuckle flower buds, in particular to a method for promoting the accumulation of luteolin in honeysuckle flower buds by using fungi. Background technique [0002] With the surge of the trend of "returning to nature", traditional natural herbal medicines are becoming more and more popular. Honeysuckle comes from Lonicera Lonicerae Lonicera japonica Thunb.'s dried flower buds or early bloomed flowers are cold in nature and sweet in taste. It has the effect of clearing heat and detoxifying, cooling and dispelling wind-heat. It is a commonly used medicine in traditional Chinese medicine. According to statistics, honeysuckle is used in about 1 / 3 of traditional Chinese medicine prescriptions, and It is widely used in Chinese patent medicines such as Shuanghuanglian, Yinqiao Jiedu Pills, and Yinhuang Oral Liquid. It has played a huge role in the prevention and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00
CPCA01G17/005
Inventor 方磊周洁张华
Owner 山东中平药业有限公司
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