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High performance liquid chromatography measurement method for measuring content of artepillin C in soft capsules of Brazilian bee propolis

A high-performance liquid chromatography, medium-atepirin technology, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve problems such as the inability to accurately determine the content of atepirin in Brazilian propolis soft capsules and the like

Inactive Publication Date: 2017-07-04
JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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Problems solved by technology

[0004] The object of the present invention is to overcome the problem that can not accurately measure the content of atipirin C in the Brazilian propolis soft capsule in the prior art, provide a kind of high performance liquid chromatography determination method of the content of atipirin C in the Brazilian propolis soft capsule

Method used

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  • High performance liquid chromatography measurement method for measuring content of artepillin C in soft capsules of Brazilian bee propolis

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Embodiment 1

[0032] A high-performance liquid chromatography assay method for measuring the content of atipirin C in Brazilian propolis soft capsules has the following steps:

[0033] (1) Weigh a capsule sample directly and place it in a homogenizer. After homogenizing at a high speed, take 1.0g of the sample into a beaker, add 5.0mL of 10% sodium hydroxide solution (by mass percentage), and place Hydrolyze in a water bath at 70°C. After the sample is completely dissolved, continue to hydrolyze for 15 minutes, cool naturally to room temperature, add hydrochloric acid to acidify, until the pH of the solution is 2.0. Then add 25mL absolute ethanol, ultrasonic extraction, extraction frequency 50Khz, temperature 40°C, extraction time 20min, transfer the sample liquid to a 50mL volumetric flask, wash the beaker twice with a small amount of ethanol, transfer it into the volumetric flask, and Dilute to volume with water and ethanol, shake well, and filter with 0.45µm filter membrane for determina...

experiment example 2

[0052] According to the steps of Example 1 of the present invention, the content of atipirin C in Brazilian propolis soft capsules stored for six months (at room temperature) was measured, and the content of atipirin C did not change significantly.

[0053] Table 1 Changes of atipirin C content in different Brazilian propolis soft capsule samples stored for 6 months, among which, sample 1 was purchased from Beijing market, sample 2 was purchased from Nanjing market, sample 3 was purchased from Nanjing market, sample 4 was purchased from Shanghai market.

[0054]

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Abstract

The invention provides a high performance liquid chromatography measurement method for measuring content of artepillin C in soft capsules of Brazilian bee propolis. The method includes the steps of: performing alkaline hydrolysis to disrupt the shells of the soft capsules, regulating the pH value to not more than 4.0 with an acidity regulator and performing ultrasonic extraction with anhydrous ethanol. The method can effectively measure the content of the artepillin C in the soft capsules of Brazilian bee propolis, and is high in accuracy and sensitivity and good in repeatability.

Description

technical field [0001] The present invention relates to a high-performance liquid chromatography determination method for the content of atipirin C in Brazilian propolis soft capsules, more specifically relates to a method for determining the content of atipirin C mainly composed of Brazilian propolis, salad oil, gelatin, glycerin, water, beeswax, egg High-performance liquid chromatography method for the content of atipirin C in Brazilian propolis soft capsules made of phospholipids and other materials. Background technique [0002] Brazilian Bee Propolis (foreign name: Brazilian Bee Propolis), refers to the propolis produced in Brazil. Because of its appearance and composition, it is quite different from the propolis in other parts of the world, and the role of Brazilian propolis is clear, and the pharmacological research is sufficient. It has attracted the attention of the world. , is the best quality propolis in the world. According to botanical origin and chemical compo...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 吴黎明仲延龙沈井旺张莉梅婷周娟
Owner JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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