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Method for increasing watermelon production by biocontrol strain Bacillus amyloliquefaciens bam22

A technology for dissolving starch spores and biocontrol strains, which is applied in the field of agricultural microorganisms, can solve the problems of bacteria producing resistance, low yield, low watermelon yield, etc., and achieves the effects of significant economic benefits, low application concentration, and low application dosage.

Active Publication Date: 2019-09-13
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The occurrence of watermelon diseases has seriously restricted the increase in watermelon production. Moreover, because watermelons have been planted year after year, and the method of repeated cropping for many years has been adopted, the disease situation in my country's watermelon field is serious. The output of existing watermelon cultivation has not been high. 20% to 30% reduction in production per household, and over 50% reduction in land with severe disease
[0004] Aiming at the problem of low yield caused by watermelon diseases, a variety of methods of combining pesticides to prevent and control diseases have been proposed at this stage, such as the use of a mixture of pesticides such as flubendin plus morpholine, triazole plus mightipeptide to prevent and control watermelon wilt , vine blight and watermelon virus disease, anthracnose and other diseases, the control effect can reach more than 85%. Difficulty, watermelon production has not been able to increase significantly
It has also been proposed that microbial agents can be used to prevent and control agricultural diseases, which has achieved a good control effect and increased production of watermelons to a certain extent, but still cannot solve the problem of low watermelon production in my country
[0005] It has been reported that using endophytic Paenibacillus polymyxa bacterium liquid to treat cotton roots can increase the yield of cotton, and using the biocontrol strain B9601-Y2 to treat cucumber, tomato and corn respectively can increase the yield by 12.6%. 24.8% and 11.5%, but it is not clear whether Bacillus amyloliquefaciens Bam22 can be used to increase watermelon production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for increasing production of watermelons by biocontrol bacterial strain Bacillus amyloliquefaciens Bam22, comprising the steps of:

[0023] 1) Take the bacterium liquid of Bacillus amyloliquefaciens Bam22 cultured for 48 hours, the initial bacterial concentration of the bacterium liquid is 3×10 9 cfu / mL, diluted 700 times with sterile water;

[0024] 2) Soak the watermelon seeds in warm water at 60°C for 18 minutes, disinfect and sterilize them, then soak the seeds with the diluted bacterial solution in step 1) for 20 hours, take them out, accelerate germination, and then sow;

[0025] 3) When transplanting watermelons, use the bacteria solution obtained in step 1) to water the roots of watermelon seedlings. The amount of bacteria solution is 200mL / plant watermelon seedlings. Water the second time every 5 days, and the amount of bacteria solution is 150mL / plant. For watermelon seedlings, water the third time every 10 days, and the dosage of bacterial solution ...

Embodiment 2

[0028] A method for increasing production of watermelons by biocontrol bacterial strain Bacillus amyloliquefaciens Bam22, comprising the steps of:

[0029] 1) Take the bacterium liquid of Bacillus amyloliquefaciens Bam22 cultured for 48 hours, the initial bacterial concentration of the bacterium liquid is 4×10 9 cfu / mL, diluted 700 times with sterile water;

[0030] 2) Soak the watermelon seeds in warm water at 55°C for 19 minutes, disinfect and sterilize them, then soak the seeds with the diluted bacterial solution in step 1) for 16 hours, take them out, germinate them, and then sow them;

[0031] 3) When transplanting watermelons, use the bacteria solution obtained in step 1) to water the roots of watermelon seedlings. The amount of bacteria solution is 180mL / strain watermelon seedlings. Water the second time every 5 days, and the amount of bacteria solution is 120mL / strain. For watermelon seedlings, pour the third time every 10 days, and the dosage of bacterial solution is...

Embodiment 3

[0034] A method for increasing production of watermelons by biocontrol bacterial strain Bacillus amyloliquefaciens Bam22, comprising the steps of:

[0035] 1) Take the bacterium liquid of Bacillus amyloliquefaciens Bam22 cultured for 48 hours, the initial bacterial concentration of the bacterium liquid is 5×10 9 cfu / mL, diluted 700 times with sterile water;

[0036] 2) Soak the watermelon seeds in warm water at 50°C for 20 minutes, disinfect and sterilize them, then soak the seeds with the diluted bacterial solution in step 1) for 15 hours, take them out, accelerate germination, and then sow;

[0037] 3) When transplanting watermelons, use the bacterial solution obtained in step 1) to water the roots of the watermelon seedlings. The dosage of the bacterial solution is 150mL / strain watermelon seedlings. Water the second time every 5 days, and the dosage of the bacterial solution is 100mL / strain. For watermelon seedlings, water the third time every 10 days, and the dosage of ba...

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PUM

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Abstract

The invention provides a method for adopting biocontrol strain bacillus amyloliquefaciens Bam22 for watermelon production increase. The method comprises the following steps that 1, a bacillus amyloliquefaciens Bam22 bacteria solution cultured for 48 h is sampled, the initial concentration of the bacteria solution is 3-5*10<9> cfu / mL, and sterile water is used for diluting the solution by 700 times; 2, watermelon seeds are soaked with warm water for 18-20 min, disinfection and sterilization are performed, then, the bacteria solution diluted in the step 1 is adopted for soaking the seeds for 15-20 h, germination acceleration is performed after the seeds are fished out, and then seeds are sown; 3, during watermelon transplanting, the bacteria solution obtained in the step 1 is adopted for performing root watering on watermelon seedlings, the consumption of the bacteria solution is 150-200 mL / watermelon seedling, watering is performed for the second time five days later, and watering is performed for the third time ten days later. The method has the advantages that the watermelon yield can be increased remarkably, the bacillus amyloliquefaciens Bam22 production increase effect is remarkable, the bacteria solution application concentration is low, and the application dosage is small.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a method for increasing watermelon production by a biocontrol bacterial strain Bacillus amyloliquefaciens Bam22. Background technique [0002] my country is a big country for watermelon planting and cultivation. It is widely planted in the north and south of my country. The main production area is Hainan Island in the south, and the main planting belt is along the Yellow River in the north. Because of its sweet fruit, watermelon can quench thirst and reduce summer heat, and is deeply loved by consumers. my country has a large consumption demand for watermelon throughout the year. Therefore, how to increase the production of watermelon has become a problem that watermelon growers are eager to solve. [0003] During the growth process of watermelon, it is easy to be infected by diseases, such as fusarium wilt, viral disease, splinter blight, anthracn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N25/00A01P21/00
CPCA01N25/00A01N63/10
Inventor 黄小琴刘勇伍文宪张蕾薛龙海周西全刘红雨尹全
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI