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A Achromobacter xylosoxidans gyp4 and its application in degrading brominated flame retardants

A brominated flame retardant and achromobacter technology, applied in the field of biological treatment of environmental pollutants, can solve the problems of not much degradation effect, insignificant and other problems

Active Publication Date: 2020-02-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, among the currently obtained microorganisms, there are not many microorganisms capable of degrading 2,2',4,4'-tetrabromodiphenyl ether, and the degradation effect is not significant

Method used

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  • A Achromobacter xylosoxidans gyp4 and its application in degrading brominated flame retardants
  • A Achromobacter xylosoxidans gyp4 and its application in degrading brominated flame retardants
  • A Achromobacter xylosoxidans gyp4 and its application in degrading brominated flame retardants

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Experimental program
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Embodiment

[0026] Separation and degradation of Achromobacter xylosoxidans GYP4 Application of 2,2′,4,4′-tetrabromodiphenyl ether:

[0027] On-site collection of farmland soil around an electronic dismantling plant heavily polluted by polybrominated diphenyl ethers in Guiyu Town, Shantou City, Guangdong Province, using 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) as the only carbon source The pure inorganic salt system domesticated the degrading bacteria, and gradually increased the concentration of BDE-47 in the system according to the gradient, so that the final concentration of BDE-47 was 5mg / L. Inorganic salt medium (MSM) composition: 1) Phosphate buffer: 8.5g KH 2 PO 4 , 21.75g ​​K 2 HPO 4 ·H 2 O, 33.4g Na 2 HPO 4 12H 2 O, 5.0gNH 4 Cl, distilled water 1L; 2) MgSO 4 Aqueous solution: 22.5g MgSO 4 ·7H 2 O, distilled water 1L; 3) CaCl 2 Aqueous solution: 36.4gCaCl 2 2H 2 O, distilled water 1L; 4) FeCl 3 Aqueous solution: 0.25g FeCl 3 ·6H 2 O. Distilled water 1L; 5) Trac...

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Abstract

The invention belongs to the technical field of biological treatment of environmental pollutants, and discloses an Achromobacter xylosoxidans GYP4 and its application in degrading brominated flame retardants. The Achromobacter xylosoxidans GYP4 was deposited in the China Center for Type Culture Collection on March 9, 2017, and the preservation number is CCTCC NO: M 2017104. The bacterial strain Achromobacter xylosoxidans GYP4 of the present invention is cultured at a pH of 3.0 to 7.0, a temperature of 25 to 35°C, and shaken at 150 r / min for 4 days. Ether degradation rate can reach more than 80%. The bacteria can be used for bioremediation of water and soil polluted by polybrominated diphenyl ethers.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to Achromobacter xylosoxidans GYP4 and its application in degrading brominated flame retardants. Background technique [0002] As a brominated flame retardant with superior performance, polybrominated diphenyl ethers (PBDEs) have been widely used in commercial products such as electronic and electrical equipment, automatic control equipment, building materials, and textiles, and are global organic pollutants that widely exist in the environment. . It has the characteristics of stable structure, difficult to degrade, environmental persistence, mobility, low water solubility, not easy to degrade, bioaccumulation, etc., and it is difficult to remove through conventional physical and chemical methods. [0003] Among the 209 PBDE congeners, 2,2′,4,4′-tetrabromodiphenyl ether has the highest toxicity and the content of 2,2′,4,4′-tetrab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/025C02F101/36
CPCB09C1/10C02F3/34C02F2101/36C12N1/205C12R2001/025
Inventor 卢桂宁黄婷林浩忠党志尹华杨琛易筱筠郭楚玲
Owner SOUTH CHINA UNIV OF TECH