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Fructus hippophae flavone and application thereof
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A technology of seabuckthorn flavonoids and seabuckthorn fruit, applied in the field of biomedicine
Inactive Publication Date: 2017-07-14
ZHEJIANG UNIV
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Abstract
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Problems solved by technology
[0007] At present, there is no relevant literature report on the use of seabuckthorn flavonoids in anti-prostate cancer
Method used
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Embodiment 1
[0035] Take 100g of dried seabuckthorn fruit, add 10 times the volume (1.0L) of 70% ethanol solution, ultrasonically assist extraction at 55°C for 1 hour, repeat 3 times, filter, combine the filtrate, distill under reduced pressure, remove ethanol, concentrate the sample, and freeze-dry to obtain Seabuckthorn fruit crude extract powder 23.6g; take 10.0g of the above seabuckthorn fruit crude extract powder, add 100mL of water to redissolve, take the supernatant and pass it through AB-8 macroporous resin, wherein, the sample loading rate is 0.2BV / h, adsorption Night. Gradient elution with 10%, 30%, and 50% ethanol in sequence, the elution flow rate is 40mL / min, a total of 1.5BV is eluted, and 50% ethanol eluted components are retained, concentrated, and freeze-dried; take 1.0g of freeze-dried powder , add 600mL of hydrolyzate, the hydrolyzate is mixed by mass concentration of 4% hydrochloric acid and methanol in a volume ratio of 1:4, and the rest is water, hydrolyzed at 70°C fo...
Embodiment 2
[0038]Take 150g of dried seabuckthorn fruit, add 10 times the volume (1.5L) of 60% ethanol solution, 60 ℃ ultrasonic-assisted extraction for 1 hour, repeat 3 times, filter, combine the filtrate, distill under reduced pressure, remove ethanol, concentrate the sample, and obtain after freeze-drying Seabuckthorn fruit crude extract powder 40.18g; Take 20.2g of the above seabuckthorn fruit crude extract powder, add 200mL of water to redissolve, take the supernatant and pass it through AB-8 macroporous resin, wherein, the sample loading rate is 0.2BV / h, adsorption Night. Gradient elution with 10%, 30%, and 50% ethanol in sequence, the elution flow rate is 40mL / min, a total of 1.5BV was eluted, and 50% ethanol eluted components were retained, concentrated, and freeze-dried; take 1.2g of freeze-dried powder , add 650mL of hydrolyzate, the hydrolyzate is prepared by mixing hydrochloric acid with a mass concentration of 6.5% and methanol at a volume ratio of 1:3, and the rest is water,...
Embodiment 3
[0041] Take 250g of dried seabuckthorn fruit, add 10 times the volume (2.5L) of 80% ethanol solution, 50 ℃ ultrasonically assisted extraction for 1 hour, repeat 3 times, filter, combine the filtrate, distill under reduced pressure, remove ethanol, concentrate the sample, and obtain after freeze-drying Seabuckthorn fruit crude extract powder 60.2g; take 30.0g of the above seabuckthorn fruit crude extract powder, add 300mL distilled water to redissolve, take the supernatant and pass it through AB-8 macroporous resin, wherein, the sample loading rate is 0.2BV / h, the adsorption one night. Gradient elution with 10%, 30%, and 50% ethanol in sequence, the elution flow rate is 40mL / min, a total of 1.5BV was eluted, and 50% ethanol eluted components were retained, concentrated, and freeze-dried; the powder obtained by freeze-drying was 2.0 g, add 1200mL of hydrolyzate, which is prepared by mixing 2% hydrochloric acid and methanol at a volume ratio of 1:5, and the rest is water. Hydroly...
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Abstract
The invention discloses fructus hippophae flavone and an application thereof. The fructus hippophae flavone is extracted from fructus hippophae. An extraction method comprises the following steps of (1) performing ultrasonic extraction on the fructus hippophae with an organic solvent so as to obtain an extract; (2) concentrating the extract, and removing the organic solvent so as to obtain a crude extract; (3) performing column chromatographic separation and purification on the crude extract so as to obtain an eluate; and (4) performing acid hydrolysis on the eluate so as to obtain the fructus hippophae flavone. The fructus hippophae flavone is obtained through the following steps of performing crude extraction on the fructus hippophae with the organic solvent, performing column chromatographic separation and purification and performing acid hydrolysis. Different extraction methods have great influence on the content of effective components in the fructus hippophae flavone. Researches prove that the fructus hippophae flavone has a certain treatment and prevention effect on prostatic cancer, and can be used for preparing medicines or health-care foods for preventing or treating the prostatic cancer.
Description
technical field [0001] The invention relates to the technical field of biomedicine, in particular to a seabuckthorn flavonoid and its application. Background technique [0002] Prostatecancer refers to epithelial malignant tumors that occur in the prostate. According to the 2004 WHO "Pathology and Genetics of Tumors of the Urinary System and Male Reproductive Organs", the pathological types of prostate cancer include adenocarcinoma (acinar adenocarcinoma), ductal adenocarcinoma, urothelial carcinoma, squamous cellcarcinoma, and adenosquamous carcinoma. . Among them, prostate adenocarcinoma accounts for more than 95%. Therefore, the so-called prostate cancer usually refers to prostate adenocarcinoma. Prostate cancer is a malignant tumor with the highest incidence rate and the second highest death rate among men in western countries. In recent years, the incidence of prostate cancer among men in our country has also increased year by year, especially as our country is ent...
Claims
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Application Information
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