Method of screening traditional Chinese medicine inhibitor against anaerobic bacteria on the basis of ZnSe nanoprobe
A technology of nano-probes and anaerobic bacteria, which can be used in the analysis of nuclear magnetic resonance, etc., and can solve the problem of the decrease of the relaxation time of nuclear magnetic resonance
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example 1
[0019] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on acne bacilli (anaerobic bacteria).
[0020] 1) Nano-ZnSe immunoprobe preparation:
[0021] ZnSe nanoparticles can be purchased from the market, or can be synthesized by physical or chemical methods with reference to literature.
[0022] Nano-ZnSe coated with silicon dioxide: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-ZnSe and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated three times to...
example 2
[0027] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on Clostridium difficile (anaerobic bacteria).
[0028] 1) Nano-ZnSe immunoprobe preparation:
[0029] ZnSe nanoparticles can be purchased from the market, or can be synthesized by physical and chemical methods with reference to literature.
[0030] Nano-ZnSe coated with silicon dioxide: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-ZnSe and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated th...
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