Method for screening anaerobic bacteria traditional Chinese medicine preparation based on TiO2 nanoprobe
A nano-probe, anaerobic bacteria technology, applied in measurement devices, instruments, analysis by nuclear magnetic resonance, etc., can solve problems such as the decrease of nuclear magnetic resonance relaxation time
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example 1
[0019] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on acne bacilli (anaerobic bacteria).
[0020] 1) Nano-TiO2 immunoprobe preparation:
[0021] TiO2 nanoparticles can be purchased from the market, or can be synthesized by physical or chemical methods with reference to literature.
[0022] Nano-TiO2 coated with silicon dioxide: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-TiO2 and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated three times to...
example 2
[0027] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on Clostridium difficile (anaerobic bacteria).
[0028] 1) Nano-TiO2 immunoprobe preparation:
[0029] TiO2 nanoparticles can be purchased from the market, or can be synthesized by physical and chemical methods with reference to literature.
[0030] Nano-TiO2 coated with silicon dioxide: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-TiO2 and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated thre...
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