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A method for analyzing nicotine optical isomers in electronic cigarette liquid by normal phase liquid chromatography-tandem mass spectrometry

A technology of normal phase liquid chromatography and optical isomers, which is applied in the analysis of materials, material separation, instruments, etc., can solve the problems of long analysis time, difficult to achieve accurate quantification, and low sensitivity, and achieves short analysis time and improved sensitivity. , the effect of high sensitivity of the method

Active Publication Date: 2019-08-30
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem of chiral chromatographic column-two-dimensional gas chromatography is that the analysis time is long; and the problem of chiral chromatographic column-liquid chromatography is that the sensitivity is low, and it is difficult to realize the low content of R-(+)-nicotine accurate quantification

Method used

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  • A method for analyzing nicotine optical isomers in electronic cigarette liquid by normal phase liquid chromatography-tandem mass spectrometry
  • A method for analyzing nicotine optical isomers in electronic cigarette liquid by normal phase liquid chromatography-tandem mass spectrometry
  • A method for analyzing nicotine optical isomers in electronic cigarette liquid by normal phase liquid chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Accurately weigh 0.10 g of e-cigarette liquid, add it to a 50 mL conical flask with a stopper, add 10 mL of racemic d 3 - Isopropanol for nicotine internal standard, racemic d 3 -The concentration of nicotine is 5 mg / mL, shake for 5 min, and take the solution for normal phase liquid chromatography-tandem mass spectrometry analysis.

[0025] (2) Instrumental analysis conditions: Normal phase liquid chromatography-tandem mass spectrometry is used for analysis, and the chromatographic column used is Daicel chiral chromatographic column CHIRALPAK ® IC, the size is 4.6 mm×250 mm, i.d. 5 µm; column temperature: 30 o C; mobile phase A: n-hexane containing 0.1% diethylamine, mobile phase B: isopropanol; 95%A+5%B isocratic elution; flow rate 1.0 mL / min; injection volume 10 μL, analysis time is 25 min. Agilent high-pressure infusion pump was used to add isopropanol post-column, and the flow rate of isopropanol was controlled to 1mL / min; and the three-way valve configured ...

Embodiment 2

[0027] Five kinds of e-cigarette liquid samples were selected, and the content of nicotine optical isomers in the samples was determined according to the analysis method described in Example 1, and the results are shown in Table 3.

[0028] Table 3 Content of nicotine optical isomers in electronic cigarette liquid (mg / g)

[0029] Sample serial number S-(-)-nicotine R-(+)-nicotine 1# 6.23 0.044 2# 8.25 0.041 3# 10.63 0.021 4# 16.56 0.066 5# 18.43 0.055

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Abstract

The invention relates to a method for analyzing nicotine optical isomers in an electronic cigarette liquid by using a positive-phase liquid chromatography-tandem mass spectrometry method. The method is characterized in that the nicotine optical isomers in the electronic cigarette liquid are separated by a chiral chromatographic column, a post-column high pressure liquid output pump is used for introducing isopropyl alcohol and separating flow, and the tandem mass spectrometry is used for analyzing. The method has the beneficial effects that (1) the nicotine optical isomers in the electronic cigarette liquid are analyzed by the positive-phase liquid chromatography-tandem mass spectrometry method, so as to separate and detect the nicotine optical isomers in the electronic cigarette liquid; (2) the post-column high pressure liquid output pump is used for introducing the isopropyl alcohol and separating flow, so that the amount of non-polarity solvent entering mass spectrometry is reduced, the amount of the isopropyl alcohol of polarity solvent is compensated, and the ionizing efficiency is improved; (3) compared with the prior art, the chromatography resolution of the two types of nicotine optical isomers is high, and the difference of retention times is 1min; the analysis time is short, and is only 17min; the sensitivity is high, and the quantitative detection of lower content of R-(+)-nicotine in the cigarette liquid can be realized.

Description

technical field [0001] The invention belongs to the technical research on the separation, analysis and detection of chiral isomers in complex samples, and specifically relates to a method for analyzing nicotine optical isomers in electronic cigarette liquid by normal phase liquid chromatography-tandem mass spectrometry, mainly using chiral The chromatographic column realizes the separation of nicotine optical isomers. The high-pressure infusion pump column is used to introduce isopropanol and split the flow. Combined with tandem mass spectrometry analysis, the S-(-)-nicotine and R- Separation and sensitive detection of (+)-nicotine. Background technique [0002] E-cigarettes are a new type of tobacco product that has developed most rapidly in recent years. It uses electric heating to atomize the liquid in the electronic cigarette into an aerosol containing solvent and nicotine for smokers to inhale. Most e-cigarette liquids are a mixture of propylene glycol (or glycerin), ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 余晶晶蔡君兰王冰秦亚琼刘克建赵晓东薛聪刘绍锋张晓兵
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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