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Application of Streptomyces roche to control of parasitic weeds

A technology of Streptomyces Loucherii and parasiticity is applied in the application field of Streptomyces Loucherii in the control of parasitic weeds, and achieves the effects of alleviating the hazard, inhibiting disease pathogens, and inhibiting germination

Inactive Publication Date: 2017-07-21
陕西博秦生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preliminary test results show that Streptomyces loucherii also has multiple functions of disease prevention and growth promotion, and has a good control effect on soil-borne root diseases, but there is no report on the control of Streptomyces loucherii.

Method used

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  • Application of Streptomyces roche to control of parasitic weeds
  • Application of Streptomyces roche to control of parasitic weeds
  • Application of Streptomyces roche to control of parasitic weeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Experiment on Inhibition of Gualiedang Seed Germination by Fermentation Broth of Streptomyces Loucherii

[0027] 1. Test method:

[0028] (1) Preparation of Streptomyces Louchei Fermentation Broth

[0029] The fermented liquid of Streptomyces Loucheri adopts the mode of known liquid shaker culture to carry out, and stoste (every milliliter of fermented liquid 3.5mg dry mycelia) is diluted 10,10 respectively 2 、10 3 、10 4 Doubling later for later use. The Gaoshi No. 1 liquid medium and the PDA liquid medium that were not inoculated with Streptomyces loucherii were used as controls, and repeated 3 times.

[0030] (2) Seed germination test

[0031] Firstly, glass fiber filter paper with a diameter of 8mm was placed in a new petri dish, then 20 μL of fermentation broth, 1 piece of pre-cultivated melon seeds and 20 μL of GR24 (0.1 mg L -1 ) sequentially added to the glass fiber filter paper. Add sterile water and GR24 (0.1mg L -1 ) instead of the treatment of the fer...

Embodiment 2

[0042] Inhibitory Test of Methanol Extract of Streptomyces Loucherii on Seed Germination of Gualedang

[0043] 1. Test method

[0044] (1) Preparation of Methanol Extract of Streptomyces Loucherii

[0045] Weigh 0.050g of Streptomyces Loucherii and place it in a 1.5mL centrifuge tube, then add 1mL of methanol, ultrasonicate for 30min and then 6000r min -1 Centrifuge for 2min. The supernatant was regarded as the stock solution, diluted 10 and 100 times for use, and repeated 3 times.

[0046] (2) Seed germination test

[0047] Carry out according to above-mentioned test method that fermented liquid suppresses the seed germination of Gua Liedang, replace fermented liquid with thalline methanol extraction liquid, and test repeats 3 times.

[0048] 2. Test results

[0049] See Table 2 for the results of the methanol extract of Streptomyces louchei inhibiting the germination of Gualedang seeds. The 50.0mg / mL, 5.0mg / mL and 0.5mg / mL methanol extracts of Streptomyces loucherii ha...

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Abstract

The invention belongs to the technical field of parasitic weed control, and particularly discloses an application of Streptomyces roche to control of parasitic weeds. The Streptomyces roche D74 is collected at China Center for Type Culture Collection on March 14th, 2016, and the collection number is CCTCC M 2016123. The Streptomyces roche can be used for remarkably inhibiting orobanche aegyptiaca seed germination.

Description

technical field [0001] The invention relates to the technical field of parasitic weed control, in particular to an application of Streptomyces loucherii in the control of parasitic weeds. Background technique [0002] The genus Orobanche spp. includes more than 200 species, many of which are root parasitic weeds of vegetables and crops. Due to the lack of leaves and chloroplasts, Redang completely depends on absorbing water and nutrients from the host, thus causing serious damage to the host plant. Melon (Phelipanche aegyptiaca Pers.) belongs to the genus Phelipanche, widely distributed in southern Europe, the Mediterranean region, northern Africa, southern Asia and China, and can be parasitic in Solanaceae, Fabaceae, Umbelliferae and Compositae, etc. crops. [0003] As the damage caused by radang to agricultural production is becoming more and more serious, and radang continues to spread and infect new areas, it is urgent to find measures that can effectively control rada...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P13/02C12R1/465
CPCA01N63/00C12N1/205C12R2001/465
Inventor 陈杰马永清薛泉宏
Owner 陕西博秦生物工程有限公司
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