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Application of triterpene from poria cocos peel in preparation of medicines for treating nephropathy

The technology of Poria cocos skin and kidney disease is applied in the application field of Poria cocos triterpenoids in the preparation of medicines for treating kidney disease, which can solve the problem of high toxicity of traditional Chinese medicine extracts, and achieve the effects of reducing urine protein content and reducing the degree of lesions.

Active Publication Date: 2017-07-25
SHANDONG ACAD OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem that the effect of current nephropathy treatment agents on some cases is still unsatisfactory, and the toxicity of traditional Chinese medicine extracts is high, the present invention provides a relatively safe and effective application of poria cocos triterpenoids in the preparation of medicaments for treating nephropathy

Method used

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  • Application of triterpene from poria cocos peel in preparation of medicines for treating nephropathy
  • Application of triterpene from poria cocos peel in preparation of medicines for treating nephropathy
  • Application of triterpene from poria cocos peel in preparation of medicines for treating nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Extraction and Preparation of Poria Cortex Triterpenes.

[0025] (1) washing Poria cocos skin with hydrochloric acid with a mass fraction of 1.5%, then washing with water to neutrality, and drying;

[0026] (2) Refluxing the Poria cocos bark with 70% ethanol solution to extract twice, each extraction time is 0.5h, the dosage of Poria cocos bark and ethanol solution is 1:10 (g / ml), combine the extracts, and extract Liquid recovery ethanol to dryness;

[0027] (3) the dry matter of step (1) is dissolved in water into a solution, adding a mixture of n-butanol and ethyl acetate with a volume ratio of 7:1.5 for extraction, the volume of the mixture of n-butanol and ethyl acetate is 2 times that of the aqueous solution, The organic phase reclaims the solvent to dryness to obtain the crude product;

[0028] (4) The crude product is dissolved with 100-120 mass times of ethanol to form a solution, and the neutral alumina chromatography column on the gained solution, ...

Embodiment 2

[0030] Example 2 Determination of active ingredients in triterpenoids of Poria cocos bark.

[0031] Hydroxy-lanoster 7,9(11), 24-triene-21-acid and dehydroibrillic acid content in the extract are determined as follows:

[0032] Instruments and reagents: Waters high-performance liquid chromatography system, Water600 pump, 996 diode array detector, M32 chromatography workstation, Lichrospher-C18 column, 200×4.6mm, 5μ (product of Jiangsu Hanbang Technology Co., Ltd.).

[0033] Reagents: acetonitrile (chromatographically pure grade), 3β-hydroxy-lanoster 7,9(11), 24-triene-21-acid and dehydroiblic acid (prepared by Shandong Traditional Chinese Medicine New Preparation Center, the content of which reached 98.0 %above).

[0034] Detection conditions: mobile phase: acetonitrile: 0.5% acetic acid water (80:20), detection wavelength 242nm.

[0035] Preparation of reference substance solution: Accurately weigh 3β-hydroxy-7,9(11),24-triene-21-acid reference substance, put it in a 10mL v...

Embodiment 3

[0038] Example 3 The efficacy of triterpenoids of poria cocos on doxorubicin nephrotic syndrome in rats.

[0039] 1 Materials and methods

[0040] 1.1 Instruments and medicines

[0041] WFZ UV-PC ultraviolet spectrophotometer (Unocal (Shanghai) Instrument Co., Ltd.); DXL-DL metabolic cage: Jiangsu Feng's Experimental Animal Equipment Co., Ltd.; calf serum albumin: Xi'an Walson Biotechnology Co., Ltd. Company; Coomassie Brilliant Blue G-250: China Pharmaceutical Group Shanghai Chemical Reagent Company; urine protein test paper: Guangdong Huadu Gaoerbao Biotechnology Co., Ltd.; doxorubicin hydrochloride: Zhejiang Hisun Pharmaceutical Co., Ltd., 10mg / bottle , batch number 130201, when used as a solvent, inject physiological saline as a solvent, and prepare a 2mg / ml solution; Poria cocos triterpenes use the sample in Example 1, and use olive oil as a solvent, and prepare a 10 mg / ml solution.

[0042] 1.2 Animals

[0043] Select healthy male Wistar rats, weighing 158 ± 13g, prov...

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Abstract

In order to solve the problems that the existing drugs for treating nephropathy are still not ideal in effect for certain cases and high in toxicity of Chinese herb extracts, the invention provides application of triterpene, which is safer and remarkable in curative effect, from poria cocos peel in preparation of medicines for treating the nephropathy; the triterpene from the poria cocos peel is an ethanol extract of the poria cocos peel. The triterpene from the poria cocos peel can reduce the pathologic change degree of the kidney, reduce the content of urinary protein caused by the nephropathy, reduce creatinine and urea nitrogen and play a role in the treatment for the nephropathy.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to the application of poria cortex triterpenes in the preparation of medicines for treating nephropathy. Background technique [0002] In recent years, with the aggravation of environmental pollution, the acceleration of people's work and life rhythm, the intensification of occupational competition, the change of diet structure and the abuse of antibiotics, the incidence of chronic nephritis has been increasing year by year. The survey results show that the prevalence of chronic kidney disease among adults in my country is 10.8%. Based on this, it is estimated that there are about 120 million chronic kidney disease patients in the population over the age of 18. Primary disease is chronic disease, and course of disease is long, and damage is big, and has a strong impact on health and work, and economic loss such as chronic nephritis medical expenses is used in my country ever...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575A61K36/076A61P13/12
CPCA61K31/575A61K36/076A61K2236/333A61K2236/35A61K2236/39A61K2236/55A61K2300/00
Inventor 闫雪生孙丹丹于蓓蓓刘瑾
Owner SHANDONG ACAD OF CHINESE MEDICINE
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