A kind of dna bar code standard detection method and its application of identifying giant clam

A barcode and clam technology, applied in recombinant DNA technology, DNA/RNA fragments, biochemical equipment and methods, etc., can solve problems such as difficult to accurately identify clam, achieve automation and standardization, break through excessive dependence, and quickly price Effect
CN106978490BActive Publication Date: 2020-12-22OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2020-12-22

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Abstract

The invention provides a DNA bar code combination for identifying tridacna. The DNA bar code combination comprises a DNA bar code for identifying hippopus, of which a nucleotide sequence is SEQ ID NO:1, a DNA bar code for identifying phosphate-free tridacna, of which a nucleotide sequence is SEQ ID NO:2, a DNA bar code for identifying tridacna gigas, of which a nucleotide sequence is SEQ ID NO:3, a DNA bar code for identifying tridacna maxima, of which a nucleotide sequence is SEQ ID NO:4, a DNA bar code for identifying phosphate tridacna, of which a nucleotide sequence is SEQ ID NO:5, and a DNA bar code for identifying tridacna crocea, of which a nucleotide sequence is SEQ ID NO:6. The invention also provides a universal primer pair for amplifying the tridacnidae shellfish DNA bar codes, and sequences of primers of the primer pair are SEQ ID NO:7 and SEQ ID NO:8. The bar codes and the amplification primers provided by the invention can effectively identify specific categories of the tridacna.
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Description

technical field

[0001] The invention belongs to the technical field of shellfish molecular markers, and in particular relates to a DNA barcode standard detection method for identifying giant clams and an application thereof. Background technique

[0002] The family Tridacnidae belongs to Bivalvia, Heterodonta, and Veneroida. It is a large bivalve mollusk that mainly lives in tropical seas, coral reefs or seas. sand surface. Tridacnaceae shellfish have delicious meat, high nutritional and medicinal value, and are expensive. Their processed products have extremely high artistic value and have been overfished all over the world. In recent years, some countries have listed three species of giant clam (Tridacna gigas) as rare and protected animals in the world for protection. Tridacna maxima is also listed as Near Threatened on the IUCN Red List. At present, 9 species of Tridacnaidae have been discovered. Different species can be distinguished according to the distribution of ...

Claims

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