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O-type and a-type bivalent synthetic peptide vaccines for bovine foot-and-mouth disease and their preparation methods and applications

A technology for synthetic peptide vaccines and bovine foot-and-mouth disease, which is applied in the direction of multivalent vaccines, biochemical equipment and methods, vaccines, etc., can solve the problems that affect the use of new vaccines, cannot effectively protect animals, and have poor effects, and achieve good immune efficacy, The effect of reliable vaccine quality and simple preparation process

Active Publication Date: 2020-08-25
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In terms of research on new vaccines for foot-and-mouth disease, there have been reports on genetically engineered subunit vaccines, foot-and-mouth disease virus vector vaccines, and genetically engineered vaccines for foot-and-mouth disease. use of vaccines
In addition, these vaccines are often less effective against currently circulating strains that have mutated and cannot effectively protect animals

Method used

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  • O-type and a-type bivalent synthetic peptide vaccines for bovine foot-and-mouth disease and their preparation methods and applications
  • O-type and a-type bivalent synthetic peptide vaccines for bovine foot-and-mouth disease and their preparation methods and applications
  • O-type and a-type bivalent synthetic peptide vaccines for bovine foot-and-mouth disease and their preparation methods and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1, the solid-phase synthesis of polypeptide antigen of bovine foot-and-mouth disease O type, A type bivalent synthetic peptide vaccine

[0064] The present invention studies the variation of the main antigenic sites of the FMD by determining the sequence of the newly popular strains of the domestic FMD and combining the sequences of the FMD vaccine strains, and counts the variation frequency of the main mutated amino acid sites, and at the same time combines computer-aided FMD Analysis and prediction of antigenic sites, chemical synthesis of possible antigenic site peptides, that is, according to the statistical variation frequency of variable sites, different amino acids are used at these sites to obtain a multiplicity covering all current possible variable sites A candidate polypeptide antigen. Furthermore, these candidate polypeptide antigens were screened through a large number of animal experiments, and the polypeptide antigens capable of inducing an im...

Embodiment 2

[0102] Embodiment 2, the preparation of bovine foot-and-mouth disease O type, A type bivalent synthetic peptide vaccine

[0103] 1.1 Preparation of antigen aqueous phase

[0104] Weigh the synthetic peptide antigens represented by polypeptide 1 or polypeptide 2 prepared according to Example 1, mix them in a molar ratio of 1:1, and then dilute the total concentration of the synthetic peptide antigens to 50 μg / ml with sterile water for injection. The resulting antigen solution was filtered through a filter with a pore size of 0.2 μm and sterilized.

[0105] 1.2 Preparation of oil phase adjuvant

[0106] Sterilize the oil phase adjuvant at 50V at 121°C for 30min, and set aside.

[0107] 1.3 Emulsion of synthetic peptide vaccine

[0108] Clean the IKA emulsification equipment with 2000ml of sterilized distilled water for 3 times, then put the oil phase into the emulsification tank at 20-28°C according to the volume ratio of the oil phase adjuvant and the antigen water phase a...

Embodiment 3

[0110] Embodiment 3, the antigen screening test of bovine foot-and-mouth disease O type, A type bivalent synthetic peptide vaccine

[0111] 1. Materials and methods

[0112] 1.1 Materials

[0113] 1.1.1 Synthetic peptide vaccines

[0114] The screening antigen of the bivalent synthetic peptide vaccine was prepared according to the method in Example 1, and then the single-component vaccines containing the screening antigen were respectively prepared according to Example 2. The vaccines prepared against the alternative antigens of O-type foot-and-mouth disease virus have corresponding batch numbers: NO1301, NO1302, NO1303, and NO1304. The vaccines prepared against the alternative antigens of type A foot-and-mouth disease virus have corresponding batch numbers: NA1401, NA1402, NA1403, NA1404, NA1405.

[0115] 1.1.2 Test animals

[0116] 45 6-month-old healthy yellow cattle [purchased from a cattle farm in Lanzhou] were selected with negative foot-and-mouth disease antibody (n...

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Abstract

The invention provides an antigen polypeptide composition and vaccine for preparing a cow foot-and-mouth disease O-type and A-type divalent synthetic peptide vaccine. The antigen peptide composition comprises polypeptides as shown in sequence 1 and sequence 2. The vaccine in the invention comprises the polypeptide composition. The cow foot-and-mouth disease O-type and A-type divalent synthetic peptide vaccine provided by the invention has good immunological efficiency, and the antigen of the vaccine is chemically synthetic peptide and does not contain foot-and-mouth disease viral nucleic acid particles, and the safety is high. Therefore, the vaccine in the invention can effectively solve antigenic variation of current foot-and-mouth disease virus, the biological safety is low, and the vaccine is easy for large-scale synthesis and has a good application prospect. The invention also provides a preparation method and pharmacy application of polypeptide and peptide vaccines.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular, the invention relates to a type O and type A bivalent synthetic peptide vaccine of bovine foot-and-mouth disease and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious, febrile infectious disease of cloven-hoofed animals, which is widely distributed in the world. Foot-and-mouth disease is highly contagious and spreads rapidly. After infecting pigs, cattle, sheep and other livestock, it will lead to the death of young animals and a sharp decline in the production capacity of adult animals. Therefore, it seriously endangers the development of animal husbandry and the production and supply of meat and animal products. At present, foot-and-mouth disease has greatly blocked and restricted the market circulation and international trade of animals and animal products, causing huge economic losses to animal husbandry pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/09A61K39/135A61K39/295A61P31/14
CPCA61K39/12A61K2039/552A61K2039/70C07K14/005C12N2770/32122C12N2770/32134
Inventor 宋芳肖进巴利民王振豹董春娜张蕾王楠齐鹏粟利芳张欣张艳宾马爱荣张爱民赵洪涛刘新月李静
Owner CHINA ANIMAL HUSBANDRY IND
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