Application of flagellin derived polypeptide for preventing and treating chemoradiotherapy-induced damage of reproductive system

A technology of flagellin and reproductive system, applied in peptide/protein components, medical preparations containing active ingredients, diseases, etc.

Inactive Publication Date: 2017-08-04
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the radiation damage protection of CBLB502 on the male reproductive system has not been reported

Method used

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  • Application of flagellin derived polypeptide for preventing and treating chemoradiotherapy-induced damage of reproductive system
  • Application of flagellin derived polypeptide for preventing and treating chemoradiotherapy-induced damage of reproductive system
  • Application of flagellin derived polypeptide for preventing and treating chemoradiotherapy-induced damage of reproductive system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Analysis of sperm count and motility in mice

[0056] The experimental animals used in the present invention are SPF grade C57BL / 6J mice, animal license number: SCXX (Beijing) 2012-0001, body weight 18-22 g, 6-8 weeks old, purchased from Beijing Weitong Lihua Co., Ltd. . The mice were housed in the Animal Experiment Center of the Academy of Military Medical Sciences, 5 per cage, in a special pathogen-free (SPF) animal room. Before the experiment, all animals were kept in the same rearing environment, room temperature was 21-25°C, humidity: 50%-60%, all experimental animals had free access to food and water, and the circadian rhythm was changed for 12 hours each.

[0057] Good sperm motility is the basis of fertilization and one of the important indicators of male fertility. The sperm motility rate is divided into 4 grades according to the World Health Organization WHO criteria; fast forward motility (grade A), slow forward motility (grade B), non-forward mot...

Embodiment 2

[0063] Example 2 Detection of sperm abnormality rate in mice

[0064] Sperm deformity rate is the most direct indicator to evaluate sperm quality. like figure 2 The types of sperm deformities shown are hook less, folded, amorphous, short tail and bananalike. The specific detection method is as follows:

[0065] Take the 5Gy-irradiated mouse semen smear in Example 1, fix it with 80% alcohol for several seconds, air dry, and stain with 1% eosin for 30 minutes; after fixation, wash with pure water for several seconds, and then air dry. For microscopic observation, at least 200 spermatozoa were observed per mouse. First, use a low-power microscope to find the part with a clear background and less overlapping sperm, and use a high-power microscope to check the sperm morphology. The types of deformities identified were classified as unhooked, banana-shaped, folded, amorphous, short-tailed, and others.

[0066] After 5Gy irradiation, the sperm deformity rate increased significa...

Embodiment 3

[0067] Example 3 Histological observation of mouse testis

[0068] 5Gy 60 Mice were sacrificed on days 1, 3, 7, 15, 30, 60 and 120 days after Co gamma irradiation. The mouse testis was taken, the organ index was analyzed, and the tissue section analysis was performed.

[0069] The experimental results see image 3 , found that on the 60th day after irradiation, the back and head of the mice had severe hair loss and skin ulceration; while the mice in the CBLB502 pretreatment group only had a small amount of hair loss ( image 3 A); On the 30th day after irradiation, the testis volume of irradiated mice became smaller and shrunken, while the degree of atrophy in the CBLB502 pretreatment group was significantly reduced ( image 3 B). The testicular organ index was 0.18%, which was less than 0.49% of the normal group of mice ( image 3 C); while the testis index of mice in CBLB502 pretreatment group was 0.25%, which was higher than that in irradiation group (p<0.05). Therefo...

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Abstract

The invention discloses a novel application of flagellin derived polypeptide. The invention provides an application of flagellin derived polypeptide in preparing a product for treating and/or inhibiting testicular injury diseases. The flagellin derived polypeptide is preferably CBLB502. The flagellin derived polypeptide CBLB502 can prevent injury of testis induced by chemoradiotherapy and can be used for preparing a drug for inhibiting injury of testis.

Description

technical field [0001] The present invention belongs to the field of biotechnology, and relates to a new use of flagellin-derived polypeptides for treating and / or inhibiting diseases of mammalian reproductive system such as testicular damage. Background technique [0002] Ionizing radiation can have direct and indirect effects on the body. As an organ with extremely active cell proliferation and differentiation, testis is highly sensitive to ionizing radiation. Studies have shown that ionizing radiation interferes with the metabolism, proliferation, and differentiation of spermatogenic cells, ultimately leading to abnormal sperm development, decreased sperm count, decreased motility, and sexual dysfunction such as infertility. 0.3Gy of γ-ray can significantly reduce spermatogonia in mice. A single dose of 0.15Gy exposure to adult males can cause oligospermia, and 0.35-0.50Gy can cause reversible azoospermia. But even restored germ cells are likely to be accompanied by gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P15/00A61P15/08
Inventor 杨晓明葛常辉詹轶群白浩王磊
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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