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Preparation method of placental cell epimatrix

A technology of extracellular matrix and placenta, which is applied in the field of preparation of placental extracellular matrix, can solve the problems of extracellular matrix biological components and physical property damage, long decellularization time, incomplete tissue decellularization, etc., and is beneficial to production expansion The effect of simplification, easy operation, and shortened decellularization time

Inactive Publication Date: 2017-08-04
同坤源(天津)医药科技有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0009] (1) Long decellularization time;
[0010] (2) Incomplete tissue decellularization;
[0012] (4) The biological components and physical properties of the extracellular matrix are damaged

Method used

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Embodiment Construction

[0036] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0037] Part I: Preparation of placental extracellular matrix

[0038] Step1: Frozen storage

[0039] The fresh placenta was quick-frozen and stored at -80°C for more than 48 hours to ensure complete freezing. Fresh placenta is taken from human body.

[0040] Step2: Melting

[0041] Remove the frozen placenta and thaw at room temperature.

[0042] Step3: grinding

[0043] The thawed placental tissue was separated and ground into tissue fragments on a grinder, the size of the tissue fragments being 0.1mm-2mm.

[0044] Step4: Cell removal, virus inactivation and rinsing

[0045] Decellularization, virus inactivation and rinsing are performed on the ground tissue fragments at room temperature. The specific process is as follows:

[0046] (1) Immerse the tissue fragments in a 1% SDS solution (the concentration of the SDS solution can range from 0.2% to 2%), and keep...

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Abstract

The invention discloses a preparation method of placental cell epimatrix. The method comprises the following steps of (1) unfreezing frozen placenta at the room temperature; (2) separating the placenta tissue; grinding the placenta tissue into tissue fragments on a grinding machine, wherein the size is 0.1mm to 2mm; (3) performing decellularization, virus inactivation and rinsing processing on the tissue fragments; (4) putting the tissue fragments into an environment being -20 DEG C to 80 DEG C over the night; or fast soaking the tissue fragments into dry ice; then, putting the tissue fragments into a freeze drying machine to be subjected to freeze drying for 24h to 48h; obtaining the placental cell epimatrix. The method has the beneficial effects that (1) three kinds of tissue organ decellularization methods are combined; simplicity and easy operation are realized; the decellularization time is greatly shortened; biological ingredients and physical performance cannot be obviously damaged; various cell reaction actors and wound healing factors are maintained to the maximum degree; rich collagen protein, elastic fiber protein and GAGs are contained; the method is beneficial to production enlargement; (2) the decellularization is complete; no allosome tissue antigen is remained.

Description

technical field [0001] The invention relates to a preparation method of placental extracellular matrix, belonging to the technical field of bioengineering. Background technique [0002] Loss of tissue and organ function is a major obstacle to human health. In theory, regeneration of tissues and organs could overcome these obstacles. [0003] The extracellular matrix (ECM) inherent in organisms can guide organ development, repair and physiological regeneration. [0004] Currently, there are three main methods for decellularization of extracellular matrix: [0005] (1) Vascular perfusion method; [0006] (2) enzymatic method; [0007] (3) Chemical decellularization agent cleaning method. [0008] These decellularization methods often have the following disadvantages: [0009] (1) Long decellularization time; [0010] (2) Incomplete tissue decellularization; [0011] (3) Allogeneic tissue antigen residues; [0012] (4) The biological components and physical properties ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3633A61L27/3687A61L27/3691A61L2430/40
Inventor 王家伦张芝加
Owner 同坤源(天津)医药科技有限公司
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