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Molecular marker for detecting resistance of cow mastitis and application of molecular marker

A technology of molecular markers and inflammation resistance, applied in the field of genetic breeding, can solve the problems of poor effect and low heritability of clinical mastitis

Active Publication Date: 2017-08-04
北京市畜牧总站
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AI Technical Summary

Problems solved by technology

[0003] The heritability of clinical mastitis is low, ranging from 0.01 to 0.10 (Chu et al., 2012), and the effect of direct selection in dairy cow breeding is poor

Method used

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  • Molecular marker for detecting resistance of cow mastitis and application of molecular marker
  • Molecular marker for detecting resistance of cow mastitis and application of molecular marker
  • Molecular marker for detecting resistance of cow mastitis and application of molecular marker

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Embodiment 1

[0023] 1 Materials and methods

[0024] 1.1 Materials

[0025] Blood samples of 1,980 Holstein cows (from 15 bull families) were collected from 5 dairy farms in the suburbs of Beijing, and the number of cows sampled from the 5 dairy farms was 450, 390, 410, 380, and 350, respectively. Jugular vein blood collection, all blood samples were 10mL / head, anticoagulated with citrate and glucose, and frozen at -20°C. Genomic DNA was extracted by phenol-chloroform extraction, dissolved in TE buffer (10mmol / L Tris-HCl (pH 8.0), 1mmol / L EDTA (pH 8.0)), and stored at 4°C. According to the somatic cell count (SCC) of the first lactation period recorded by DHI data of dairy cows, use the formula SCS=log 2 (SCC / 10000)+3 to calculate the somatic score.

[0026] 1.2 Screening of BRCA1 gene exon 14 and flanking sequence SNP polymorphisms

[0027] Based on the number of somatic cells in the first fetus of participating dairy cows, the individuals with extreme mastitis in dairy cows were scre...

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Abstract

The invention provides a molecular marker for detecting the resistance of cow mastitis and application of the molecular marker. Cows are preferable Holstein cows. The molecular marker is a B allele on a 50439ins T site of an intron 14 of a BRCA1 gene; the 50439ins T of the BRCA1 gene of the Holstein cow is a newly-discovered mutant site; and three genotypes are totally detected, wherein an AA genotype is a dominant genotype, and both scores of an AB genotype and BB genotype somatic cells are lower than that of the AA genotype (P is smaller than 0.01). The results show that the B allele on the 50439ins T site of the BRCA1 gene can be used as the molecular marker for the resistance of the cow mastitis.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to the selection, determination and application of a molecular marker for detecting cow mastitis. Background technique [0002] Cow mastitis is the first major disease affecting the development of dairy industry, and genetic factors play an important role in the occurrence of mastitis. Searching for mastitis resistance genes or molecular markers in dairy cows for marker-assisted selection has become a research hotspot in dairy cow disease-resistant breeding. [0003] The heritability of clinical mastitis is low, ranging from 0.01 to 0.10 (Chu et al., 2012), and the effect of direct selection in dairy cow breeding is poor. The somatic cell count (SCC) or somatic cell score (SCS) heritability of dairy cows is 0.05-0.29 (Rupp et al., 1999; Heringstad et al., 2006), and it is easy to record during production. Therefore, SCS and SCC are currently commonly used for indirect sel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 杨宇泽冯涛
Owner 北京市畜牧总站
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