Method for measuring chilling requirement of cherries through semi-vivo culture

Abstract

The invention provides a method for measuring the chilling requirement of cherries through semi-vivo culture. The method comprises cherry branches are collected; the cherry branches are culture at low temperature; the cherry braches are culture at room temperature, the flower bud germination rate and the leaf bud germination rate are counted, and the chilling requirement of the cherries is measured; according to the method for collecting the cherry branches, the base portions of the branches are pruned flat, the length of the branches are controlled to be 30-50 cm, and at least five flower buds are reserved in each branch. The low-temperature culture and the room-temperature culture are used alternately, so that external conditions are simulated as far as possible, the temperature is effectively controlled, the branches are avoided from being affected by the uncontrollable factor, namely external temperature, or the external temperature is recorded by means of other devices, and convenience is brought to the calculation of the chilling requirement.

Description

technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a method for measuring the cooling capacity of cherries by semi-living culture. Background technique [0002] Cooling requirement refers to the effective low temperature hours required for natural dormancy of deciduous fruit trees. Most deciduous fruit trees need to accumulate a certain amount of cooling required to break dormancy and heat up. The cooling requirement is of great significance to the suitable planting of deciduous fruit trees, protected cultivation and forecasting of climate change. [0003] Cherry fruit is bright in color and beautiful in appearance. It is rich in sugar, protein, vitamins, calcium, iron, phosphorus, potassium and other elements. It is a high-grade fruit with high economic value and is suitable for our country. Facility cultivation can advance the maturity period of fruit, thereby significantly improving economic benefits, but cherries, l...

AI Technical Summary

Problems solved by technology

[0004] Most of the existing methods for measuring the cooling capacity of fruit trees are to directly collect branches from the field at regular intervals after the leaves are fallen, and then bring them back to the laboratory for light cultivation, observe the germination rate of flower buds or leaf buds, and then according to the temperature monitored by the local meteorological bureau or automatically The temperature measured by the monitor is used to measure and calculate. This method takes a long time, and the branches are easily affected by uncontrollable external temperature factors. At the same time, other equipment needs to be used to record the external temperature.

Liu Rendao (Liu Rendao, Liu Jianjun. Research on the amount of cooling needed for different varieties of sweet cherries. Northern Horticulture, 2009(2): 84-85) and others collected the branches and put them directly in a 4°C refrigerator for storage, and then cultured them at room temperature. The disadvantage of the method is that the branches will dry out or become moldy when stored for a long time, and the storage in the refrigerator is always in a dark environment, which will have a certain impact on the differentiation of flower buds, and the amount of cooling required cannot be accurately measured.

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