Polygonatum cyrtonema immature embryo culture micro-stem tuber breeding method
A technology for culturing Polygonatum polyflora and young embryos, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions to achieve the effects of vigorous vitality, high survival rate and large multiplication coefficient
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Embodiment 1
[0016] The microtuber propagation method of Polygonatum multiflora immature embryo culture comprises the following steps:
[0017] (1) Selection of excellent individual plants of Polygonatum multiflora: Select a single plant of Polygonatum multiflora with strong growth, strong disease resistance, large rhizomes, good quality and excellent agronomic characteristics, observe its flowering and fruiting, and test the fruit 1 month before the fruit matures. , Harvest the immature fruit of a single plant.
[0018] (2) Extraction of immature seeds and immature embryos: The immature fruit of a single plant of Polygonatum multiflora is disinfected according to the routine tissue culture explant disinfection procedure. In a sterile environment, the fruit is opened, the seeds are taken out, and the seeds are peeled off. Take the young and complete embryos from the seeds.
[0019] (3) Induction culture of immature embryos: insert aseptically stripped young embryos into induction growth m...
Embodiment 2
[0025] The microtuber propagation method of Polygonatum multiflora immature embryo culture comprises the following steps:
[0026] (1) Selection of excellent individual plants of Polygonatum polyflora: Select a single plant of Polygonatum polyflora with robust growth, strong disease resistance, large rhizomes, good quality and excellent agronomic characteristics, observe its flowering and fruiting, and 2 months before the fruit matures , Harvest the immature fruit of a single plant.
[0027] (2) Extraction of immature seeds and immature embryos: The immature fruit of a single plant of Polygonatum multiflora is disinfected according to the routine tissue culture explant disinfection procedure. In a sterile environment, the fruit is opened, the seeds are taken out, and the seeds are peeled off. Take the young and complete embryos from the seeds.
[0028] (3) Induction culture of immature embryos: insert the aseptically stripped young embryos into induction growth medium MS+2.0m...
Embodiment 3
[0034] The microtuber propagation method of Polygonatum multiflora immature embryo culture comprises the following steps:
[0035] (1) Selection of excellent individual plants of Polygonatum multiflora: Select a single plant of Polygonatum multiflora with strong growth, strong disease resistance, large rhizomes, good quality and excellent agronomic characteristics, observe its flowering and fruiting, and test the fruit 1 month before the fruit matures. , Harvest the immature fruit of a single plant.
[0036] (2) Extraction of immature seeds and immature embryos: The immature fruit of a single plant of Polygonatum multiflora is disinfected according to the routine tissue culture explant disinfection procedure. In a sterile environment, the fruit is opened, the seeds are taken out, and the seeds are peeled off. Take the young and complete embryos from the seeds.
[0037] (3) Induction culture of immature embryos: insert the aseptically stripped young embryos into induction grow...
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