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Compound microbial inoculant special for lilium davidii var. unicolor and preparation method of compound microbial inoculant

A compound microbial agent, lily technology, applied in the field of agricultural microorganisms, can solve the problems of fertility decline, environmental pollution, soil compaction, etc., and achieve the effects of inhibiting growth, improving ecological environment, and improving biological yield and quality

Active Publication Date: 2017-08-11
甘肃绿能农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fertilizers used by Lanzhou lily are mainly chemical fertilizers, which cause problems such as soil compaction and fertility decline; the prevention and control of plant diseases and insect pests mainly uses chemical pesticides, which not only easily produce drug resistance, but also cause serious environmental pollution. There is no compound microbial agent specially designed for Lanzhou lily

Method used

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  • Compound microbial inoculant special for lilium davidii var. unicolor and preparation method of compound microbial inoculant
  • Compound microbial inoculant special for lilium davidii var. unicolor and preparation method of compound microbial inoculant
  • Compound microbial inoculant special for lilium davidii var. unicolor and preparation method of compound microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Composition of special compound microbial agent for Lanzhou lily:

[0038] Bacillus thuringiensis strain SMs13 2.3×10 9 cfu / mL;

[0039] Bacillus licheniformis strain SMrs15 2.5×10 9 cfu / mL;

[0040] Bacillus Laterosporus strain LEs15 6.1×10 9 cfu / mL;

[0041] Bacillus amyloliquefaciens strain LEr08 4.2×10 9 cfu / mL;

[0042] Bacillus megaterium strain FBs03 4.6×10 9 cfu / mL;

[0043] Bacillus subtilis strain FBrs14 6.4×10 9 cfu / mL.

[0044] The preparation method of the compound microbial inoculum for Lanzhou lily includes the following steps:

[0045] 1) Inclined cultivation: the strain SMs13 ( Bacillus thuringiensis ), strain SMrs15 ( Bacillus jicheiformis ), strain LEs15 ( Bacillus laterosporus ), strain LEr08 ( Bacillus amyloliquefciens ), strain FBs03 ( Bacillus megaterium ) And strain FBrs14 ( Bacillus subtilis ) Six kinds of microbial strains were respectively inoculated on solid medium to activate the strains;

[0046] 2) Primary seed culture: Inoculate the activ...

Embodiment 2

[0053] Composition of special compound microbial agent for Lanzhou lily:

[0054] Bacillus thuringiensis strain SMs13 8.2×10 8 cfu / mL;

[0055] Bacillus licheniformis strain SMrs15 8.6×10 8 cfu / mL;

[0056] Bacillus Laterosporus strain LEs15 8.9×10 8 cfu / mL;

[0057] Bacillus amyloliquefaciens strain LEr08 9.0×10 8 cfu / mL;

[0058] Bacillus megaterium strain FBs03 9.2×10 8 cfu / mL;

[0059] Bacillus subtilis strain FBrs14 8.8×10 8 cfu / mL.

[0060] The preparation method of the bacterial agent is the same as in Example 1. The difference is that:

[0061] In step 1), inoculate the six strains on NA solid medium at 20°C and culture for 3 days;

[0062] In step 2), the six strains were inoculated in NA liquid medium and cultured on a shaker for 3 days at 20°C; counted with a hemocytometer, the concentration of each strain reached 1.0×10 8 cfu / mL or more;

[0063] In step 3), the inoculum amount is 0.5% of the volume of the liquid medium;

[0064] In step 3), the mixed fermentation culture con...

Embodiment 3

[0066] Composition of special compound microbial agent for Lanzhou lily:

[0067] Bacillus thuringiensis strain SMs13 1.1×10 10 cfu / mL;

[0068] Bacillus licheniformis strain SMrs15 1.0×10 10 cfu / mL;

[0069] Bacillus Laterosporus strain LEs15 1.4×10 10 cfu / mL;

[0070] Bacillus amyloliquefaciens strain LEr08 1.3×10 10 cfu / mL;

[0071] Bacillus megaterium strain FBs03 1.3×10 10 cfu / mL;

[0072] Bacillus subtilis strain FBrs14 1.5×10 10 cfu / mL.

[0073] The preparation method of the bacterial agent is the same as in Example 1. The difference is that:

[0074] In step 1), inoculate the six strains on NA solid medium at 30°C and culture for 2 days;

[0075] In step 2), six strains were inoculated in NA liquid medium and cultured on a shaker for 3 days at 30°C; counted with a hemocytometer, the concentration of each strain reached 1.0×10 8 cfu / mL or more;

[0076] In step 3), the inoculum amount is 2% of the volume of the liquid medium;

[0077] In step 3), the mixed fermentation culture cond...

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Abstract

The invention discloses a compound microbial inoculant special for lilium davidii var. unicolor. The compound microbial inoculant is fermentation liquor containing 8.0x10<8>-1.5x10<10> cfu / mL bacterial strain Bacillus thuringiensis SMs13, 8.0x10<8>-1.5x10<10> cfu / mL bacterial strain Bacillus Jicheiformis SMrs15, 8.0x10<8>-1.5x10<10> cfu / mL bacterial strain Bacillus laterosporus LEs15, 8.0x10<8>-1.5x10<10> cfu / mL bacterial strain Bacillus amyloliquefciens LEr08, 8.0x10<8>-1.5x10<10> cfu / mL bacterial strain Bacillus megaterium FBs03, and 8.0x10<8>-1.5x10<10> cfu / mL bacterial strain Bacillus subtilis FBrs14. The invention further discloses a preparation method of the compound microbial inoculant. The special compound microbial inoculant can coordinate the nutrition, promote the growth of lilium davidii var. unicolor, and improve the quality, yield as well as disease resistance and stress resistance of lilium davidii var. unicolor, and thus the purpose of improving the property of soil is achieved.

Description

Technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and relates to a special composite microbial inoculum for Lanzhou lily and a preparation method thereof. Background technique [0002] Lanzhou Lily( Lilium davidii var. unicolor ) Is a variant of Sichuan lily in our country. It is a high-quality product among edible lilies. It combines nutritional, medicinal and health care values. It is one of the famous and special vegetables in the country. Lanzhou lily is big, plump, beautiful in color, beautiful in shape, and rich in nutrients. It has the best quality among all edible lilies in our country. Lanzhou lily planting has become a characteristic advantageous industry and a pillar industry of the local farmers' economy, and an important source of income for farmers. [0003] With the growing area of ​​lily cultivation, chemical fertilizers and chemical pesticides are used in large quantities and varieties. While chemical fertilizers ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G13/00A01B79/02C05G3/04C05G3/02C05G3/00A01N63/00A01P21/00A01P7/00A01P1/00A01P3/00C12R1/07C12R1/10C12R1/125C05G3/60C05G3/80
CPCA01B79/02A01G13/00A01N63/00C05G3/00C05G3/60C05G3/80C12N1/20A01N2300/00
Inventor 秦波杨晓燕金辉燕志强李秀壮
Owner 甘肃绿能农业科技股份有限公司
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