Microbial film for determining biochemical oxygen demand and preparation method of microbial film
A biochemical oxygen demand and microbial film technology, applied in the field of biochemistry, can solve problems such as single structure, cumbersome production, and secondary pollution of water bodies, and achieve the effects of accelerating the reaction process, shortening detection time, and avoiding secondary pollution
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Embodiment 1
[0038] This embodiment provides a microbial film for measuring biochemical oxygen demand and a preparation method thereof. The microbial film is rGO / PPy / B.subtilis microbial film, and the preparation principle is as follows: figure 1 As shown, the preparation process is as follows (parameters are shown in Table 1):
[0039] 1. Prepare 1mg / L GO solution, add Py to 10mL GO solution to 1mM, and mix well by ultrasonication for 1h; pour the mixed solution into a hydrothermal reaction kettle and react at a constant temperature of 180°C for 12h to obtain black columnar rGO / Py Hydrogel composites;
[0040] 2. The rGO / Py hydrogel was used as the working electrode, the platinum electrode was used as the counter electrode, the Ag / AgCl electrode was used as the reference electrode, and the electrolyte solution was 0.005M PBS solution (pH 7.0). Polymerization of Py, timing potential 0.8V, timing time 30min, to obtain rGO / PPy composite material; freeze and dry rGO / PPy to obtain three-dimen...
Embodiment 2
[0043]In this example, the rGO / PPy / B.subtilis microbial film was still prepared, using the same preparation method as in Example 1, except that the condition parameters were different, as shown in Table 1.
Embodiment 3
[0045] In this example, the rGO / PPy / B.subtilis microbial film was still prepared, using the same preparation method as in Example 1, except that the condition parameters were different, as shown in Table 1.
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