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A combined vaccine, -RSV technology, applied in the biological field, can solve the problems of mucosal and cellular immune response and limited immune protection effect, lack of mastery of VLP technology, imbalance of G protein vaccine, etc. and economical burden, the effect of easy operation
Active Publication Date: 2017-08-18
BRAVOVAX
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In 2009, the Sandbulte MR research team used virus-like particles (VLP) containing only influenza virus NA for the detection of anti-NA antibodies. This method has applied for a patent. Due to the limited yield of the prepared VLP, there may also be baculovirus in the system. It is difficult for laboratories or vaccine manufacturers who lack the knowledge of VLP technology to carry out
The research of RSV vaccine has a
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Embodiment 1
[0059] 1. Preparation of Silicate Nanoparticles
[0060] There are many kinds of nanoparticles, and the preparation process of one of them is selected for illustration in this embodiment.
[0061] Silicate nanoparticles, raw materials are easy to obtain, the preparation process is relatively simple, and it is convenient to use as a carrier after modification. The preparation process of silicate among the present invention is specifically as follows:
[0062] s1: Put silicic acid in absolute ethanol to form a silicic acid alcohol solution with a concentration of 0.3mol / L, then add the dispersant medical grade polyvinylpyrrolidone (PVP), and the content of the dispersant in the system after adding is silicic acid 0.8, ultrasonic stirring, ultrasonic frequency 15kHz, ultrasonic stirring adopts sealed ultrasonic reaction, and the reaction time is 60min;
[0063] Adding the dispersant to ethanol can have good solubility, and at the same time, silicic acid is suspended in ethanol....
Embodiment 2
[0084] The difference between this example and Example 1 is that the preparation conditions of the nanoparticle carrier and the protein antigen are different, as follows:
[0085] s1: Put silicic acid in absolute ethanol to form a silicic acid alcohol solution with a concentration of 0.5mol / L, then add the dispersant medical grade polyvinylpyrrolidone (PVP), and the content of the dispersant in the system after adding is silicic acid 0.3, ultrasonic stirring, ultrasonic frequency 10kHz, ultrasonic stirring adopts sealed ultrasonic reaction, and the reaction time is 45min;
[0086] s2: Use a rotary evaporator to distill the suspension of s1 under reduced pressure at a pressure of 0.03MPa until a viscous slurry is formed, and then subject the viscous slurry to 60°C 1.5Hz micro-sonication until it is completely evaporated to dryness to form a dispersion Sexual particles;
[0087] s3: Add the dispersible granules to the reactor, add the catalyst and ammonia water, seal the reacti...
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Abstract
The invention discloses a modified seasonal flu-RSV (Respiratory Syncytial Virus) combined vaccine and a preparation method thereof. The modified seasonal flu-RSV combined vaccine is prepared from a flu protein antigen, an RSV protein antigen and a nanoparticle carrier, wherein the surface of the nanoparticle carrier is hydroxylated; the flu protein antigen and the RSV protein antigen react with hydroxyl groups on the surface of the nanoparticle carrier and are connected to the surfaces to nanoparticles. According to the modified seasonal flu-RSV combined vaccine disclosed by the invention, the nanoparticles are used as a novel protein carrier, antigen protein is efficiently and stably connected, and the nanoparticles having a flu antigen and an RSV antigen at the same time are successfully constructed; an experiment on mice verifies that a good systemic immune response can be caused, the nanoparticles are used as the carrier and can be repeatedly synthesized, the stability is high, not only can digestion of all kinds of enzymes in a body be resisted, but also the modified seasonal flu-RSV combined vaccine has many advantages of tolerating high pressure, sterilizing and the like. By adopting the nanoparticles as the novel protein carrier, the method is simple, the operation is simple and convenient, various protein and polysaccharide antigens can be connected, too much modification is not needed, and a good application prospect is obtained.
Description
technical field [0001] The invention relates to a modified seasonal influenza-RSV combined vaccine and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] 1. Pathogenic microorganisms and vaccines [0003] Microorganisms that can cause infectious diseases in humans or animals are called pathogenic microorganisms or pathogenic microorganisms. Infection refers to the process of pathogenic microorganisms invading the body, growing and multiplying in certain parts, and causing a series of pathophysiological processes. When pathogenic microorganisms invade the body, the pathogenic microorganisms interact with the body and change each other's activity and function. Therefore, whether they can cause infectious diseases depends on the pathogenicity or virulence of the pathogenic microorganisms on the one hand, and on the other hand. Depends on the body's resistance, that is, immunity. The ability of pathogenic bacteria to cause inf...
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