Hybridoma cell strain, anti-CRH antibody C-D17 based on hybridoma cell strain, and application of anti-CRH antibody C-D17 to detection
A hybridoma cell line and hybridoma cell technology are applied in the field of anti-adrenocorticotropin-releasing hormone monoclonal antibodies, which can solve problems such as health threats to rescue workers in plateau work, and achieve high sensitivity, low material consumption, and good specificity. Effect
Inactive Publication Date: 2017-08-18
陈锋 +1
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The invention relates to the field of biotechnology, and discloses an anti-CRH antibody C-D17 of a hybridoma cell strain, and application of the anti-CRH antibody C-D17 to detection. The hybridoma cell strain is preserved in China Center for Type Culture Collection on May 31, 2016 with the preservation number of CCTCC NO. C2015215, and is classified and named hybridoma cell C-D17. The monoclonal antibody C-D17 based on anti-cortin releasing hormone (CRH) generated by the hybridoma cell strain, and application of the monoclonal antibody to a kit for detecting acute altitude sickness are also provided. The anti-CRH monoclonal antibody C-D17 can recognize the C-end epitope of CRH, and has high specificity and high titer which can reach 1:4x10<6>. The double-antibody sandwich ELISA kit for detecting CRH has high specificity and sensitivity.
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