Establishing method of paper-based photoinduced electrochemical biological sensor with high flux

A photoelectrochemical and biosensor technology, used in material analysis by observing the influence of chemical indicators, material analysis by optical means, and chemical reaction of materials, etc., can solve the problem of expensive instruments and complicated operations. , limit wide application and development and other issues, to achieve the effect of improving electrode conductivity, saving detection costs, and improving detection sensitivity

Inactive Publication Date: 2017-08-18
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods are expensive, complicated to operate, and even require professionals to operate, which is not conducive to the realization of portable high-throughput rapid on-site detection, which limits its wide application and development.

Method used

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  • Establishing method of paper-based photoinduced electrochemical biological sensor with high flux
  • Establishing method of paper-based photoinduced electrochemical biological sensor with high flux
  • Establishing method of paper-based photoinduced electrochemical biological sensor with high flux

Examples

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Embodiment 1

[0036] Example 1: Simultaneous detection of MCF-7, K562 and HL-60 cancer cells in human blood.

[0037] (1) Design the hydrophobic wax batch printing pattern of the microfluidic paper chip on the computer, the style is as attached figure 1 As shown, the wax batch printing pattern consists of four parts, which are working chip (A), hollow channel chip (B), semi-hydrophilic channel chip (C) and auxiliary chip (D), 1 is the solution inlet, 2- 4 is the working electrode 1-3, 5 is the hollow channel, 6-8 is the semi-hydrophilic channel, 6 is printing 60% wax, 7 is printing 40% wax, 8 is printing 80% wax, and 9 is the auxiliary electrode printing area; The attached figure 1 Punch and cut the middle grid area to prepare a hollow channel; place the chromatographic paper printed with hydrophobic wax batch printing patterns in an oven, and heat at 130 °C for 30 s to make the wax penetrate into the paper fiber and build a completely hydrophobic and semi-hydrophobic part; Print carbon e...

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Abstract

The invention discloses a photoinduced electrochemical biological sensor with advantages of simple operation and high flux, which is successfully applied to simultaneously detect three types of cancer cells. The photoinduced electrochemical biological sensor is characterized in that a layer of gold nanoparticles is modified onto the surface of paper fiber through gold nanometer self-catalytic reduction; then, preparing cadmium sulfide-graphene-zinc oxide bar ternary composite materials at the surface of a paper working electrode which are modified by long gold, and using as photo-electrodes; using chemical light emitting to replace the traditional xenon lamp; by controlling the chemical light emitting reaction sequence, sequentially exciting photo-electrodes; sequentially detecting three photo-current peaks, so as to simultaneously detect the three types of cancer cells.

Description

technical field [0001] The invention relates to the field of paper-based high-throughput photoelectrochemical analysis and detection, and more specifically relates to a construction method of a paper microfluidic chip analysis and detection platform based on a chemiluminescent photoelectrochemical analysis method. Background technique [0002] Cancer is one of the most important diseases that threaten human health today. Early diagnosis of cancer is one of the effective ways to improve the survival rate of patients. Cancer cells are a kind of mutated cells, which are the source of cancer. Cancer cells are different from normal cells. They have three characteristics: unlimited proliferation, transformation and easy transfer. They can proliferate indefinitely and destroy normal cell tissues. Therefore, it is of great significance to construct a fast and accurate method for the analysis and detection of cancer cells. So far, various analysis methods have emerged one after anot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/77G01N27/327G01N33/574G01N33/531
CPCG01N21/77G01N27/327G01N33/531G01N33/574G01N2021/7753
Inventor 王衍虎孔庆坤葛慎光于京华颜梅
Owner UNIV OF JINAN
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