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Method for determining content of calycosin-7-glucoside in radix astragali seu hedysari by ASE-HPLC (Accelerated Solvent Extraction-High Performance Liquid Chromatography) method

A technology of isoflavone and glucoside, applied in the field of chemical detection, can solve the problems of high professional level of technicians and long reflux time, etc.

Inactive Publication Date: 2017-08-18
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] This method takes a long time to reflux and has a high level of professionalism for technicians

Method used

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  • Method for determining content of calycosin-7-glucoside in radix astragali seu hedysari by ASE-HPLC (Accelerated Solvent Extraction-High Performance Liquid Chromatography) method
  • Method for determining content of calycosin-7-glucoside in radix astragali seu hedysari by ASE-HPLC (Accelerated Solvent Extraction-High Performance Liquid Chromatography) method
  • Method for determining content of calycosin-7-glucoside in radix astragali seu hedysari by ASE-HPLC (Accelerated Solvent Extraction-High Performance Liquid Chromatography) method

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Embodiment 1

[0027] 1. Instruments and reagents

[0028] 1.1 Instruments:

[0029] Electronic analytical balance (XA205DU), ASE350 fast solvent extractor (DIONEX, USA), ThermoU3000 UHPLC liquid chromatograph

[0030] 1.2 Reagents:

[0031] Water: Class I water in accordance with GB / T 6682;

[0032] Methanol (CH 4 O): chromatographically pure (for liquid chromatography);

[0033] Acetic acid (CH 3 COOH): analytically pure.

[0034] 1.3 Materials

[0035] Astragalus (produced in Inner Mongolia, batch number 1511100; produced in Gansu, batch number 15081901; produced in Inner Mongolia, batch number 150718) were purchased from Wuzhou or Yulin medicinal material market, and were identified by Huang Heng, deputy director of Guangxi Wuzhou Food and Drug Inspection Institute.

[0036] The reference substance calycocetin glucoside was provided by the China Institute for the Control of Biological Products, batch number: 111920-201505.

[0037] 2. Method

[0038] 2.1 Preparation of reference...

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Abstract

The invention discloses a method for determining the content of calycosin-7-glucoside in radix astragali seu hedysari by an ASE-HPLC (Accelerated Solvent Extraction-High Performance Liquid Chromatography) method. The method has the advantages of less time consumption for extraction and high detection precision and belongs to the field of chemical detection. The method comprises the following steps: extracting radix astragali seu hedysari powder by adopting an ASE method and collecting a methanol extracting solution; determining the content of the calycosin-7-glucoside in the methanol extracting solution by adopting an HPLC method. The method can be used for replacing a pharmacopoeia method to extract the calycosin-7-glucoside in the radix astragali seu hedysari and detect the content of the calycosin-7-glucoside.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to an ASE-HPLC method for measuring the content of calycosin glucoside in Astragalus membranaceus. Background technique [0002] "Chinese Pharmacopoeia" 2015 Edition Part One Preparation method for the test product: Preparation of the test solution Take about 1g of the powder of this product (passed through a No. 4 sieve), weigh it accurately, put it in a round bottom flask, add 50ml of methanol precisely, and weigh it Weight, heat to reflux for 4 hours, let cool, weigh again, use methanol to make up the lost weight, shake well, filter, accurately measure 25ml of the filtrate, recover the solvent to dryness, add methanol to dissolve the residue, transfer to 5ml Bottle, add methanol to the mark, shake well, that is. [0003] The method has a longer reflux time and has a higher professional level for technicians. Contents of the invention [0004] In view of the above deficiencie...

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N2030/062G01N2030/884
Inventor 廖强陈学松韦日伟王丽丽欧妮
Owner WUZHOU INST FOR FOOD & DRUG CONTROL