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Regeneration construction method based on pulp-dentin complex and biological scaffold material for constructing pulp-dentin complex structure

A construction method and composite technology are applied in the field of bio-scaffold materials for constructing dental pulp-dentin complex structure, which can solve the problems such as the lack of biological scaffold materials and the inability to form a dental pulp-dentin complex structure, and improve the preparation time. , Residual reduction, the effect of promoting regeneration

Active Publication Date: 2017-08-29
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the construction of the pulp-dentin complex is the key to the study of pulp tissue regeneration, and it is not yet possible to form a complete pulp-dentin complex structure.
Although a lot of research has been devoted to how to construct a complete pulp-dentin complex structure, there is still no method that can completely construct the pulp-dentin complex when the pulp tissue regenerates
[0003] And there is no such kind of bio-scaffold material for pulp-dentin complex

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] This embodiment provides a regeneration construction method based on the pulp-dentin complex as follows:

[0058] The fresh porcine-derived dental pulp tissue was completely taken out, sliced ​​into slices, ultrasonically cleaned, SDS, TritonX-100, ultrasonically cleaned again, nucleic acid tissue was removed, DPEM was further ground, and sterilized in double antibody. Obtain DPEM injectable material; resuspend the centrifuged dental pulp stem cells, mix with DPEM injection material and mix well; implant laminin and DPEM+cell as a complex into the pulp cavity to realize the pulp-dentin complex final regeneration.

Embodiment 2

[0060] This embodiment provides a regeneration construction method based on the pulp-dentin complex as follows:

[0061] The fresh porcine-derived dental pulp tissue was completely taken out, sliced ​​into slices, ultrasonically cleaned, SDS, TritonX-100, ultrasonically cleaned again, nucleic acid tissue was removed, DPEM was further ground, and sterilized in double antibody. Obtain DPEM injectable material; resuspend the centrifuged mesenchymal stem cells, mix them with DPEM injection material and mix well; implant FGF and DPEM+cell into the pulp cavity as a composite to realize the pulp-dentin complex eventually regenerated.

Embodiment 3

[0063] This embodiment provides a regeneration construction method based on the pulp-dentin complex as follows:

[0064] The fresh porcine-derived dental pulp tissue was completely taken out, sliced ​​into slices, ultrasonically cleaned, SDS, TritonX-100, ultrasonically cleaned again, nucleic acid tissue was removed, DPEM was further ground, and sterilized in double antibody. Obtain DPEM injectable material; resuspend the centrifuged dental pulp stem cells, mix with DPEM injection material and mix well; implant FGF and DPEM+cell into the pulp cavity as a composite to achieve the final pulp-dentin complex regeneration.

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Abstract

The invention relates to the field of oral medicine, in particular to a regeneration construction method based on pulp-dentin complex and a biological scaffold material for constructing a pulp-dentin complex structure. The method comprises the following steps: firstly preparing a dental pulp extracellular matrix material; secondly mixing dental pulp extracellular matrix injection material with stem cell, so as to obtain a mixed material; finally compounding the mixed material with protein factors. A regeneration complex structure of the pulp-dentin complex is obtained, and complete regeneration of the pulp-dentin complex is realized. The acellular preparation time during DPEM preparation is improved by preparing the DPEM into an injectable material, and the residual amount of heterologous nucleic acid is further reduced. Growth factors are added on a pulp-dentin juncture surface in a DPEM injection material, and the adhesion, migration and distribution of cell are effectively regulated and controlled, so that the regeneration of the pulp-dentin complex is promoted, and the complete regeneration of dental pulp is realized.

Description

technical field [0001] The invention relates to the field of stomatology, in particular to a regeneration construction method based on a pulp-dentin complex and a biological scaffold material for constructing a pulp-dentin complex structure. Background technique [0002] At present, tissue engineering method is an ideal technical means to realize the regeneration of dental pulp tissue in the future. Extracellular matrix materials derived from xenogeneic pulp tissue are considered to be effective scaffold materials for promoting regeneration of dental pulp tissue. The porcine-derived dental pulp extracellular matrix material (dental pulp extracellular matrix, DPEM) has good revascularization potential and can effectively promote the regeneration of dental pulp tissue. However, the construction of the pulp-dentin complex is the key to the study of pulp tissue regeneration, and it is not yet possible to form a complete pulp-dentin complex structure. Although a lot of research...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/36A61L27/38A61L27/54
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/3834A61L27/40A61L27/54A61L2300/252A61L2300/412A61L2300/414A61L2430/12
Inventor 陈岗黄海涛权慧欣肖迪陈剑锋于勇
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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