Polypeptide with antibacterial anti-inflammatory activity and application thereof

An active and inflammatory reaction technology, applied in the field of molecular biology, can solve the problems of low content of active polypeptide Lunasin, low activity, unfavorable separation and purification, etc., and achieve the effect of easy separation and purification, high activity, and efficient expression

Active Publication Date: 2017-09-19
广东尚品汇生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the naturally isolated active polypeptide Lunasin has the defects of low content and low activity, which is not conducive to separation and purification, which limits its application

Method used

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  • Polypeptide with antibacterial anti-inflammatory activity and application thereof
  • Polypeptide with antibacterial anti-inflammatory activity and application thereof
  • Polypeptide with antibacterial anti-inflammatory activity and application thereof

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Embodiment Construction

[0019] The present invention will be described in detail below with reference to the drawings and specific embodiments, but it should not be understood as a limitation of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0020] The present invention provides a polypeptide with antibacterial and anti-inflammatory activity (hereinafter referred to as G-Lunasin). The nucleotide sequence of the polypeptide is shown in SEQ ID NO. 1, and the amino acid sequence is shown in SEQ ID NO. 2. Polypeptide is an improvement on the amino acid structure of the active polypeptide Lunasin. Adding amino acid residues to the Lunasin amino acid sequence shown in SEQ ID NO. 3 increases the activity of the original Lunasin and its expression efficiency in cells, and reduces its separation and purificat...

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Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to polypeptide with antibacterial anti-inflammatory activity and application thereof. The nucleotide sequence of the polypeptide is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2. The polypeptide with the antibacterial anti-inflammatory activity is obtained through modification on the basis of a polypeptide Lunasin amino acid structure, and can be efficiently expressed in cells; the separation and the purification are easy; the activity is higher.

Description

Technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a polypeptide with antibacterial and anti-inflammatory activity and its application. Background technique [0002] Antibacterial peptides are a class of small molecule defense peptides synthesized by organisms. They have broad-spectrum bactericidal effects. They have strong killing effects on both gram-negative and gram-positive bacteria. They are particularly effective against certain fungi and mycoplasma. Drug-resistant bacteria also have a good killing effect and are an important member of the animal's innate immune system. General antibiotics work by blocking the biosynthesis of biological macromolecules, and antibacterial peptides can perforate the bacterial cell membrane to form ionic channels, causing structural damage to the bacterial cell membrane, causing a large amount of intracellular water-soluble substances to leak out, and finally leading to The ba...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/70C12N15/29C12N1/21A61K38/16A61P31/04
CPCA61K38/00C07K14/415C12N15/70
Inventor 强黎明卢奎董雪茹吕名秀李玥
Owner 广东尚品汇生物科技有限公司
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