Fruit and vegetable juice processing method by radio frequency coupling high-voltage pulse electric field processing
A high-voltage pulsed electric field and processing method technology, applied in the fields of food electrical treatment, food heat treatment, food science, etc., can solve the problems of high environmental protection pressure, water and energy consumption, etc. The effect of reducing flavor loss and quality degradation
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[0020] (1) Preparation of polyphenol oxidase crude enzyme solution: a 100g apple sample was quickly removed from the stem and nucleus, then cut into small pieces, and poured into a tissue masher together with about 400ml of acetone pre-cooled to -18℃ ( 20000rpm, 1min), and then quickly filter with suction to retain the precipitated part on the filter paper, and blow off the residual acetone in the thin layer of precipitation with cold air until the precipitate has no acetone smell. The obtained acetone powder was transferred to 100 mL of 0.05 mol / L phosphate buffer (pH 6.5) and stirred for 30 min, then centrifuged (4° C., 10000 r / min, 20 min), and the supernatant obtained by centrifugation was a crude polyphenol oxidase enzyme solution.
[0021] (2) Determination of the activity of polyphenol oxidase: add 0.5mL crude enzyme solution and 2.5mL 0.2mol / L catechol solution (pH 6.5) into the cuvette and quickly shake it and put it into the spectrophotometer. The change in absorbance o...
Example Embodiment
[0023] Example 1
[0024] The radio frequency device with the publication number CN104782758A is used to process apple tissue; among them, the radio frequency device includes a conveying device and a radio frequency device with an upper plate and a lower plate. The upper plate is located above the conveying device and the material conveyed by the conveying device is on the upper The electrode plate and the lower electrode plate move between the upper electrode plate and the lower electrode plate, and high-frequency current flows through the upper electrode plate and the lower electrode plate. Therefore, after the upper electrode plate and the lower electrode plate pass high-frequency current, there is a radio frequency between the two, and the conveying device moves the animal material between the upper electrode plate and the lower electrode plate.
[0025] Specific steps are as follows:
[0026] (1) Pretreatment: Choose fresh red Fuji apples, wash them with water, peel them, and s...
Example Embodiment
[0037] Example 2
[0038] The application of radio frequency heating treatment device (invention patent application with publication number CN104782758A) is used to process apple tissue, the specific steps are as follows:
[0039] (1) Pretreatment: Choose fresh red Fuji apples, wash them with water, peel them, and slice them. The slice thickness is 10mm.
[0040] (2) Radio frequency processing: Place the cut apple slices on the conveyor belt in the center of the lower electrode plate of the radio frequency processing chamber. The operating frequency of the radio frequency processing device is 27.12MHz, the power is set to 3.5kW, and different electrode plate spacing is set, and the sample thickness is 10mm, set different processing time, control the auxiliary temperature in the radio frequency processing chamber to 55℃, the auxiliary temperature is provided by hot air ≥55℃, and an external hot air generator can be used to collect ambient air and pass it through a 5.6kW electric heate...
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