Production method of feed additive rich in heat-resistant protease and Cordycepin by means of solid state fermentation with Cordyceps militaris

A technology for solid-state fermentation and Cordyceps militaris, which is applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of improving utilization, enhancing immunity, and eliminating nutritional inhibitory factors.

Active Publication Date: 2017-09-22
XUZHOU HONGYU AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development and utilization of Cordyceps products as feed additives has not been reported abroad. In terms of domesti

Method used

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  • Production method of feed additive rich in heat-resistant protease and Cordycepin by means of solid state fermentation with Cordyceps militaris
  • Production method of feed additive rich in heat-resistant protease and Cordycepin by means of solid state fermentation with Cordyceps militaris

Examples

Experimental program
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Effect test

Example Embodiment

[0030] Example 1. Screening of Cordyceps militaris strains

[0031] Isolation and screening of the above-mentioned Cordyceps militaris strains:

[0032] 1. Isolation and screening of strains

[0033] In August 2015, wild Cordyceps militaris was collected in Yimeng Mountain, Yinan County, Shandong Province. The collection site was a forest with loose soil and rich humus. The ambient temperature was 20-28°C and the air humidity was 70-80%. A total of 9 wild Cordyceps strains were collected, and the Cordyceps was slowly peeled off the layer, and the surface of the Cordyceps was rinsed repeatedly with clean water until it was cleaned. Then use sterile filter paper to absorb moisture on the surface of Cordyceps. Use 75% ethanol or 0.1 liter of mercury to disinfect the surface, and rinse again with sterile water. Then, carefully cut out a small piece from the cordyceps militaris, place it on the cordyceps activated solid medium, and culture it at 22-26°C. After the hyphae grow out, obs...

Example Embodiment

[0057] Example 2: Compound mutagenesis and breeding method of Cordyceps militaris strain

[0058] (1) Strain activation culture The wild strain XZCM6 isolated in Example 1 was transferred to the PDA solid slant medium for activation culture, and cultured at a constant temperature of 25-28°C for 4-5 days. The strain was activated twice as described above.

[0059] (2) Preparation of strain mycelium Take 1cm from the activated mycelium solid plate medium 2 The mycelium block was inoculated into a 250mL Erlenmeyer flask containing 40 mL of PDA mycelium culture medium, and cultured at 24~28℃ and 160~200 r / min under constant temperature shaking for 3~7 days. Mycelium liquid culture medium formula: 20~30g glucose, 5~8g peptone, 200ml 20% potato extract, (NH 4 ) 2 S0 4 3~5g, KH 2 P0 4 1~2g, MgS0 4 ·7H 2 0 1~2g, 4~6g yeast powder, add water to make the volume to 1L, pH 6.5~7.0, sterilize at 121℃ for 20min.

[0060] (4) Protoplast preparation MgS0 4 ·7H 2 0. Mannitol is dissolved in double...

Example Embodiment

[0064] Example 3 Preparation of solid-state fermentation strains

[0065] This example 3 illustrates the method for preparing the heat-resistant protease and cordycepin feed additive by using Cordyceps militaris HYCM12 as the strain solid-state fermentation method, including the following steps:

[0066] Step 1. Strain activation: First, take the preserved Cordyceps militaris HYCM12 test tube strain and inoculate it on a culture dish activation medium, culture it in an incubator at 20-26°C in the dark for 5-7 days to obtain first-class seeds.

[0067] Activated slant medium: 200 g potato, washed and chopped, added 1000 mL water and boiled and kept for 20 minutes, filtered with gauze, and the filtrate was then added 20 g glucose, 1 mL inorganic salt solution, and 15 g agar. Continue to boil, fully dissolve, and pack into test tubes , Each test tube is about 4 mL, the culture medium is sterilized at 121°C, and the sterilization time is 20 minutes. After the sterilization is completed, ...

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Abstract

The invention relates to a preparation method of a Cordyceps feed additive. According to the method, Cordyceps militaris which produces heat-resistant protease and Cordycepin in high yield is used as a solid state fermentation production strain. Through the process steps of preparation of a fermentation strain, solid state fermentation with conical flasks, solid state fermentation with koji trays, proteolysis after fermentation and the like, the Cordyceps feed additive is prepared. The protein enzyme activity in dry culture obtained by the solid state fermentation with the koji trays reaches 12941 U/g, and the Cordycepin content is 780 mg/100 g. According to the feed additive prepared from fermentation products, the active functional components of Cordyceps are maintained to the utmost extent, besides, the feed additive also contains highly active heat-resistant protease, functional active polypeptides and amino acids, wherein the functional active polypeptides and the amino acids are obtained by hydrolysis of the protease. By means of the additive, the growth of animals can be better promoted, and the conversion rate of the feed is improved, so that the cost is reduced; moreover, the immunity and disease resistance of the animals can be improved, and the survival rate is improved, so that the cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of processing Cordyceps militaris, in particular to a feed additive method for producing heat-resistant protease and cordycepin by solid-state fermentation of Cordyceps militaris. Background technique [0002] In recent years, with the continuous improvement of people's living standards, the demand for livestock products is also increasing. However, due to the keen pursuit of the output of animal products, the quality and safety of animal products are often overlooked, especially the problem that the residues of veterinary drugs and drug additives in animal products are harmful to human health. Therefore, green and natural feed additives, such as natural non-toxic effects, no drug residues, no drug resistance, can promote animal growth, can effectively and economically improve animal production performance, improve feed utilization and animal product quality, and have high breeding benefits. This type of fe...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/40C12N9/58A23K50/30A23K10/12A23K10/37C12R1/645
CPCC12N1/14C12N9/58C12P19/40C12N1/145C12R2001/645Y02P60/87
Inventor 高兆建杨杰李同祥曹泽虹李勇沈彬彬曹建冬顾强
Owner XUZHOU HONGYU AGRI TECH
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