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Cordyceps militaris polypeptide and preparation method thereof

A technology of Cordyceps militaris and aqueous solution, which is applied in the field of peptides, can solve the problems of less research on protein peptides, and achieve the effects of short rehydration time, good water solubility, and high purity

Pending Publication Date: 2017-09-29
吉林善肽堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many research reports on nucleosides and polysaccharides in Cordyceps militaris, but there are still few studies on its protein peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of a kind of Cordyceps militaris polypeptide of the present embodiment, comprises the following steps:

[0030] S1, drying and pulverizing Cordyceps militaris, weighing 100g of Cordyceps militaris powder, adding the weighed Cordyceps militaris powder into distilled water, boiling for 1 hour (continuous stirring during the boiling process), cooling to room temperature to obtain an aqueous solution of Cordyceps militaris;

[0031] S2, adjusting the pH value of the Cordyceps militaris aqueous solution to 5, and then adding a compound enzyme to enzymolyze the protein and cellulose in the Cordyceps militaris aqueous solution to obtain an enzymatic hydrolysis solution;

[0032] S3, performing filtration and decolorization treatment on the enzymolysis solution to obtain a decolorization solution, which is sequentially subjected to membrane filtration, concentration and spray drying to obtain the Cordyceps militaris polypeptide.

[0033] The enzymatic hyd...

Embodiment 2

[0038] The preparation method of a kind of Cordyceps militaris polypeptide of the present embodiment, comprises the following steps:

[0039] S1, drying and pulverizing Cordyceps militaris, weighing 100g of Cordyceps militaris powder, adding the weighed Cordyceps militaris powder into distilled water, boiling for 1.2h (continuously stirring during the boiling process), cooling to room temperature, and obtaining an aqueous solution of Cordyceps militaris;

[0040] S2, adjusting the pH value of the Cordyceps militaris aqueous solution to 6, and then adding a compound enzyme to enzymolyze the protein and cellulose in the Cordyceps militaris aqueous solution to obtain an enzymatic hydrolysis solution;

[0041] S3, performing filtration and decolorization treatment on the enzymolysis solution to obtain a decolorization solution, which is sequentially subjected to membrane filtration, concentration and spray drying to obtain the Cordyceps militaris polypeptide.

[0042] The enzymati...

Embodiment 3

[0047] The preparation method of a kind of Cordyceps militaris polypeptide of the present embodiment, comprises the following steps:

[0048] S1, dry and pulverize Cordyceps militaris, weigh 100g of Cordyceps militaris powder, add the weighed Cordyceps militaris powder into distilled water, boil for 1.4h (continuous stirring during the boiling process), cool to room temperature, and obtain an aqueous solution of Cordyceps militaris;

[0049] S2, adjusting the pH value of the Cordyceps militaris aqueous solution to 7, and then adding a compound enzyme to enzymolyze the protein and cellulose in the Cordyceps militaris aqueous solution to obtain an enzymatic hydrolysis solution;

[0050] S3, performing filtration and decolorization treatment on the enzymolysis solution to obtain a decolorization solution, which is sequentially subjected to membrane filtration, concentration and spray drying to obtain the Cordyceps militaris polypeptide.

[0051] The enzymatic hydrolysis in S2 is ...

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PUM

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Abstract

The invention relates to a cordyceps militaris polypeptide and a preparation method thereof. The preparation method comprises the following steps: S1) drying and crushing cordyceps militaris and then adding into distilled water, cooking for 1-2h, and then cooling to room temperature, thereby obtaining a cordyceps militaris aqueous solution; S2) regulating the pH value of the cordyceps militaris aqueous solution to 5-9, and then adding a compound enzyme for performing enzymolysis on the protein and cellulose in the cordyceps militaris aqueous solution, thereby obtaining an enzymatic hydrolysate; and S3) performing filtering decolorizing treatment on the enzymatic hydrolysate so as to obtain a decolorized solution, and then sequentially performing membrane filtration, concentration and spray-draying on the decolorized solution, thereby obtaining the cordyceps militaris polypeptide. According to the preparation method provided by the invention, the polypeptide matter in the cordyceps militaris can be effectively extracted, the active polypeptide in the cordyceps militaris can be effectively utilized, and the extracting efficiency is high.

Description

technical field [0001] The invention relates to the technical field of polypeptides, in particular to a polypeptide of Cordyceps militaris and a preparation method thereof. Background technique [0002] Cordyceps militaris and Cordyceps sinensis belong to different species and are very close to the medicinal ingredients and pharmacological functions of Cordyceps sinensis. They belong to the family Ergotaceae and are recognized as fungi with high medicinal value at home and abroad. Cordyceps militaris has anti-tumor, improving immune function, anti-aging and related effects in cardiovascular and other aspects. There are many research reports on the nucleosides and polysaccharides in Cordyceps militaris, but there are few researches on its protein peptides. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a Cordyceps militaris polypeptide and a preparation method thereof for the deficiencies of the prior art. [0...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 李俊伟
Owner 吉林善肽堂生物科技有限公司
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