Disease marker and application

A technology of markers and species, applied in the direction of bioreactor/fermenter combination, microorganism, specific-purpose bioreactor/fermenter, etc., can solve the problems of intestinal microbial markers in patients without ankylosing spondylitis

Active Publication Date: 2017-09-29
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports of gut microbial markers in patients with ankylosing spondylitis

Method used

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  • Disease marker and application
  • Disease marker and application
  • Disease marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] In this example, the inventors carried out an association analysis study of the whole intestinal flora microbes from 73 ankylosing spondylitis patients and 83 healthy controls' stool samples to describe the characteristics of the fecal microbial community and functional components. In general, the inventor downloaded about 428.09Gb of high-quality sequencing data (LC healthy people) and 294Gb of high-quality sequencing data obtained from experimental sequencing to construct an ankylosing spondylitis reference gene set, and compared with the downloaded LC gene set, IGC Gene sets build a more complete gene set. Metagenome analysis showed that 17 microbial species were closely related to AS disease, 7 of which were enriched in the gut microbes of healthy people, and 10 of which were enriched in the gut microbes of AS patients.

[0074] 1. Acquisition of sequencing data

[0075] 1.1 Sample collection and DNA extraction

[0076] Patients with ankylosing spondylitis came fr...

Embodiment 2

[0104] In order to confirm that the analysis results in Example 1 can be used as an ankylosing spondylitis species marker, further compare the species abundance of 31 healthy people and 24 ankylosing spondylitis patients in the verification group, and mark the final microbial species Items are screened based on validation. The acquisition and processing of the sequencing data of the verification population is carried out with reference to Example 1.

[0105] The verification results are as follows: Among the 14 species enriched in the healthy population, 7 of them obtained high-quality verification in the verification set (p_values<0.05), and the p-value and q-value of the verification of the microbial species markers enriched in healthy people As shown in Table 2 below. The microbial species were Lachnospiraceae bacterium 6_1_63FAA, Fusobacterium mortiferum, [Bacteroides] pectinophilus, Enterobacter cloacae, Eubacterium eligens, Lachnospiraceae (Lachnospiraceae bacterium 8_...

Embodiment 3

[0116] Forty-five stool samples were used to test the individual status of the sample source.

[0117] Determine actinomycetes (Actinomyces sp.ICM47), Bifidobacterium breve (Bifidobacterium breve), Bifidobacterium (Bifidobacterium sp.12_1_47BFAA), Bifidobacterium longum (Bifidobacterium sp. longum), Collinsella aerofaciens, Bifidobacterium bifidum, Bifidobacterium pseudocatenulatum, Eggerthella lenta and Bifidobacterium catenulatum) to determine whether the abundance of these nine strains in each sample falls within the 95% confidence interval of the abundance in the disease control group or healthy control group, and determine whether the abundance of these nine strains falls The state of the individual corresponding to the sample in the corresponding interval of the disease group is the patient with ankylosing spondylitis, and the state of the individual corresponding to the sample that determines that the abundance of the nine bacterial species falls into the corresponding ...

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Abstract

The invention discloses an ankylosing spondylitis microbial marker, which is formed by the following microbes: Actinomyces sp. ICM47, Bifidobacterium breve, Bifidobacterium sp.12_1_47 BFAA, Bifidobacterium longum, Collinsella aerofaciens, Bifidobacterium bifidum, Bifidobacterium pseudocatenulatum, Eggerthella lenta and Bifidobacterium catenulatum. Compared with healthy population, the ankylosing spondylitis microbial marker is obviously enriched in ankylosing spondylitis patient population, and can be used as a distinction marker of the healthy population and the ankylosing spondylitis patient population.

Description

technical field [0001] The present invention relates to the field of biomarkers, specifically, the present invention relates to disease markers and their applications, more specifically, the present invention relates to a microbial marker for ankylosing spondylitis, uses of markers for ankylosing spondylitis, a method for using ankylosing spondylitis Method for determining the state of an individual with ankylosing spondylitis markers, a device for determining the state of an individual using ankylosing spondylitis markers, a method for classifying a plurality of individuals using ankylosing spondylitis markers, a method for treating ankylosing spondylitis Medicine for ankylosing spondylitis and a method for preparing medicine for treating ankylosing spondylitis. Background technique [0002] Ankylosing spondylitis (AS) is a chronic progressive inflammatory disease that mainly invades the spine and involves the sacroiliac joints and surrounding joints. The onset is mostly m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/68C12M1/34C12Q1/02C12R1/04C12R1/01
CPCC12Q1/025C12Q1/06
Inventor 邵铁娟温成平谢志军李海昌胡长峰
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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