Method for establishing regeneration system of Peucedanum praeruptorum plants

A method of establishing a method and a technology of Qianhu, applied in plant regeneration, botanical equipment and methods, gardening methods, etc., can solve rare problems, achieve good growth status, avoid species degradation and genetic variation, and low cost Effect

Active Publication Date: 2017-10-03
JIANGSU UNIV
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Problems solved by technology

The use of tissue culture technology can inherit the excellent traits of the female parent, avoid species degeneration and genetic variation, and provide the possibility of high reproductive rate for industrial seedling cultivation of Peucedanum. At present, there are few related reports on tissue culture

Method used

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  • Method for establishing regeneration system of Peucedanum praeruptorum plants
  • Method for establishing regeneration system of Peucedanum praeruptorum plants
  • Method for establishing regeneration system of Peucedanum praeruptorum plants

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, but the protection scope of the present invention is not limited thereto.

[0031] Detailed implementation process and content of the present invention are as follows:

[0032] (1) Sterilization of explants: use the roots, stems, and leaves of the plants of Peucedaneum chinensis in good growth as explant materials, sterilize with 75% ethanol for 30 seconds, rinse with sterile water for 3 to 5 times; then use 0.1% HgCl 2 Disinfect the roots, stems, and leaves for 15, 12, and 10 minutes respectively, rinse them with sterile water for 3 to 5 times, blot the water on the surface of the roots, stems, and leaves with sterile filter paper, and cut the roots into 0.5 cm with a sterile scalpel. 3 cubes, the stems are cut into small pieces with a length of 1.0cm, and the leaves are cut into 0.25cm 2 of blocks.

[0033] In this embodiment, use 75% ethano...

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Abstract

The invention discloses a method for establishing a regeneration system of Peucedanum praeruptorum plants, belonging to the technical field of plant tissue culture. The method comprises the following steps: disinfecting Peucedanum praeruptorum explants; then inoculating callus induction mediums with the Peucedanum praeruptorum explants and carrying out callus induction and callus differentiation culture; then selecting cluster buds of the explants and carrying out rooting culture; and finally subjecting tissue culture seedlings to domestication and transplanting and establishing the regeneration system of Peucedanum praeruptorum plants. According to the invention, disinfection effect on the explants is good, and the survival rates of the explants are high; calluses are high in induction rate, great in size and good in growth state; the cluster buds are high in propagation coefficient; the tissue culture seedlings are high in rooting rate and high in survival rate after transplanting, and culture conditions for the tissue culture seedlings are not influenced by insect diseases and changes in the weather; and the method is low in cost, economic, practical, good in repeatability, short in production and growth period and applicable to industrial seedling production of Peucedanum praeruptorum.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for establishing a plant regeneration system, in particular to a method for establishing a plant regeneration system for Radix Peaches. Background technique [0002] Qianhu was first recorded in "Bielu of Famous Doctors", and it has a long history of medication for more than 1,500 years. Ningguo, Anhui is famous for its rich authentic medicinal herb Peucedanum, which is famous for its large black skin, long strips, yellow flesh, soft texture, and strong smell. It enjoys the reputation of "Ning Qianhu" in the field of traditional Chinese medicine. Due to successive years of excavation, the wild resources are gradually depleted. In the late 1990s, the Ningguo Pharmaceutical Bureau led the experiment of remodeling the wild Peucedanum to domestic species and achieved success. At present, the artificially cultivated Peucedanum has a serious branching phenomenon, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴沿友吴沿胜邢德科于睿黎明鸿姚香平徐小健
Owner JIANGSU UNIV
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