A kind of method of producing porcine pseudorabies live vaccine with passage cell line
A technology of passaging cells and cells, which is applied in the field of producing porcine pseudorabies live vaccines by using passaging cell lines, can solve the problems of easy carrying of exogenous viruses, etc., facilitate transportation and storage, have high stability, and overcome low virus titers high effect
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Embodiment 1
[0027] Embodiment 1 A kind of method of producing porcine pseudorabies live vaccine with passage cell line
[0028] The method for producing porcine pseudorabies live vaccine with the passage cell line is divided into the following steps:
[0029] S1. Passaging and culturing of Vero cells for seedling production: Digest the passaging cells with EDTA-trypsin cell dispersion liquid, continue to culture with cell growth medium, and form a monolayer for continued passaging or virus inoculation;
[0030] The cell growth liquid consists of the following components and their contents: 89% of DMEM liquid, 3% of Sapindus saponin and 8% of newborn bovine serum; and the pH value is 7.2;
[0031] S2. Propagation of Vero cell virus species: inoculate the well-grown above-mentioned passaged cell monolayer with the attenuated pseudorabies virus strain, and continue to cultivate with cell maintenance medium; harvest the cell culture venom after 3 days as the virus species for production;
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Embodiment 2
[0036] Embodiment 2 A kind of method of producing porcine pseudorabies live vaccine with passage cell line
[0037] The method for producing porcine pseudorabies live vaccine with the passage cell line is divided into the following steps:
[0038] S1. Passaging and culturing of Vero cells for seedling production: Digest the passaging cells with EDTA-trypsin cell dispersion liquid, continue to culture with cell growth medium, and form a monolayer for continued passaging or virus inoculation;
[0039] The cell growth liquid includes the following components and their contents: 90% of DMEM liquid, 1% of Sapindus sapinsin and 9% of newborn bovine serum;
[0040] S2. Propagation of Vero cell virus species: inoculate the well-grown above-mentioned passaged cell monolayer with the attenuated pseudorabies virus strain, and continue to cultivate with cell maintenance medium; harvest the cell culture venom after 3 days as the virus species for production;
[0041] The cell maintenance ...
Embodiment 3
[0045] Embodiment 3 A kind of method of producing porcine pseudorabies live vaccine with passage cell line
[0046] The method for producing porcine pseudorabies live vaccine with the passage cell line is divided into the following steps:
[0047] S1. Passaging and culturing of Vero cells for seedling production: Digest the passaging cells with EDTA-trypsin cell dispersion liquid, continue to culture with cell growth medium, and form a monolayer for continued passaging or virus inoculation;
[0048] The cell growth liquid includes the following components and their contents: 85% of DMEM liquid, 5% of Sapindus saponin and 8.7% of neonatal bovine serum;
[0049] S2. Propagation of Vero cell virus species: inoculate the well-grown above-mentioned passaged cell monolayer with the attenuated pseudorabies virus strain, and continue to cultivate with cell maintenance medium; harvest the cell culture venom after 3 days as the virus species for production;
[0050] The cell maintenance ...
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