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A kind of iridium complex with tubulin recognition function and its preparation method and application

A technology of tubulin and iridium complexes, applied in the field of tubulin probes, to achieve good results, cheap and easy-to-obtain raw materials, and high commercial value

Active Publication Date: 2019-05-31
合肥欧申福德生物科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, triplet luminescent iridium complex probes targeting tubulin have not been reported

Method used

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  • A kind of iridium complex with tubulin recognition function and its preparation method and application
  • A kind of iridium complex with tubulin recognition function and its preparation method and application
  • A kind of iridium complex with tubulin recognition function and its preparation method and application

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Embodiment Construction

[0041] The structural formula of the iridium complex (Ir-Tb) with tubulin recognition function of the present invention is:

[0042]

[0043] The preparation method of the iridium complex with tubulin recognition function of the present invention comprises the following steps:

[0044] Step 1: Synthesis of Intermediate M

[0045] pass N 2 Under gas protection, 0.60 g (2.2 mmol) of ligand L was dissolved in 20 mL of a mixed solvent of ethylene glycol monoethyl ether and distilled water (3:1, v / v), and added to a 100 mL Shrek bottle wrapped in tin foil after dissolving. , and then add 0.33 g (1.0 mmol) of iridium chloride trihydrate solution dissolved in 10 mL of water, and reflux at 110 ° C for 24 h; after the reaction, cool to room temperature, filter and wash the precipitate with a small amount of distilled water, and dry in a vacuum drying box for 12 h to obtain The orange solid powder is the intermediate M.

[0046]Ligand L was synthesized with reference to literature...

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Abstract

The invention discloses an iridium complex with a microtubulin identifying function as well as a preparation method and application of the iridium complex. The structural formula of the iridium complex with the microtubulin identifying function is shown in the description. A benzothiazole derivative is selected as a main ligand to adjust the complex to illuminate, a conjugated system of the complex is expanded by introducing phenanthroline, and the iridium complex Ir-Tb is designed and synthesized. Found by researches, the complex has a relatively large Stoke's shift and long fluorescent lifetime, a target molecule Ir-Tb can target microtubulin in a live cell, different periods of cell mitosis are observed by marking a microtubule structure, and the compound has a wide application prospect in the research field of bioscience.

Description

technical field [0001] The present invention relates to a tubulin probe, in particular to an iridium complex with tubulin recognition function and a preparation method and application thereof. Background technique [0002] Microtubules are a major component of the cytoskeleton of all eukaryotic cells, and are composed of tubulin and tubulin-binding proteins, which are linked together by α-tubulin and β-tubulin dimer. Tubulin is a dynamic cytoskeletal protein whose assembly dynamics give it a unique role in cell division. Especially during cell mitosis, under the regulation of centrosomes, microtubules can further aggregate to form spindles and guide cell differentiation from metaphase to anaphase. Since the reorganization of the microtubule network is an essential process of the cell life cycle and cell division function, the abnormal content of tubulin and the destruction of normal arrangement and aggregation are all related to the canceration of cells. It has been repor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F15/00C09K11/06G01N21/64
CPCC07F15/0033C09K11/06C09K2211/185G01N21/6402G01N21/6428G01N2021/6439
Inventor 田肖和唐义雯王景行解清园田玉鹏张琼周虹屏吴杰颖
Owner 合肥欧申福德生物科技有限责任公司
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