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CD2 binding protein (CD2AP) and protein interacting therewith

A technology of individual and serial number, applied in recombinant DNA technology, stably introducing foreign DNA into chromosomes, drug combination, etc., can solve problems such as incomplete understanding of host proteins

Active Publication Date: 2017-10-13
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no evidence of a direct interaction between dynein and NS5A
Therefore, the host proteins that interact with NS5A during transport are still incompletely understood.

Method used

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  • CD2 binding protein (CD2AP) and protein interacting therewith
  • CD2 binding protein (CD2AP) and protein interacting therewith
  • CD2 binding protein (CD2AP) and protein interacting therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0124] 1. Materials and methods

[0125] 1.1 Cell lines and viruses

[0126] Human hepatoma cells Huh7, its derived Huh7-Lunet and Huh7.5.1 cells, and HEK293T cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Gibco, #11965-092, USA), supplemented with 3.17g / l sodium bicarbonate, 10% fetal bovine serum (Gibco, #10099-141), 3g / L HEPEs, 100U / ml of penicillin and streptomycin. CON1 cells carrying the HCV 1b subgenomic HCV replicon pFKI389neo / NS3-3', derived from Huh7-Lunet, were maintained in the same medium as Huh7-Lunet cells, supplemented with 0.5 mg / ml G418 (Merck, 345810) (42 ). The infectious HCV JFH1 virus contains the full-length cDNA sequence of the HCV genotype 2a strain genome (43). The HCV J399EM virus was derived from the JFH-1 virus, and the EGFP gene was inserted after 399 amino acids of NS5A, and five adaptive mutations were introduced into the JFH1 genome to enhance virus production capacity (44). The JFH1 Luc reporter virus was provided by Profe...

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Abstract

The invention discloses a method for regulating expression of an individual CD2AP down. The method comprises the following step of applying CD2AP down-regulation compatibility to the individual, wherein the CD2AP down-regulation compatibility works through siRNA / shRNA, Crispr / cas9, crispr / cpf1, Talen or ZFNs, and therefore, the expression of the CD2AP in a liver tissue of the individual is regulated down.

Description

technical field [0001] The present invention relates to CD2-associated protein (CD2AP) and its interacting proteins, especially the interaction between CD2AP and hepatitis C virus (HCV) non-structural protein NS5A, between CD2AP and insulin receptor substrate 1 (IRS1) , and the interaction between Cbl-b / Cbl and IRS1, and further relate to reagents and methods for downregulating CD2AP expression, reagents and methods for manipulating the interaction between CD2AP and HCV NS5A to inhibit HCV assembly, manipulating CD2AP A reagent and method for treating patients with type 2 diabetes through the interaction between Cbl-b / Cbl and IRS1, and a reagent and method for treating patients with type 2 diabetes by manipulating the interaction between Cbl-b / Cbl and IRS1. In addition, the present invention also relates to CD2AP-based reagents and methods for detecting fatty liver and type 2 diabetes in serology and bile. Background technique [0002] Hepatitis C virus (HCV), a member of t...

Claims

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Application Information

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IPC IPC(8): C12N15/90C12N9/22G01N33/68G01N33/571A61K45/00A61K31/7105A61K48/00A61P3/10A61P1/16A61P31/14
CPCA61K31/7105A61K45/00C12N9/22C12N15/907G01N33/571G01N33/68G01N2333/70507G01N2800/042
Inventor 李朝阳张会侠
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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