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Enzyme hydrogel particle and preparation method thereof

A technology of gel particles and enzyme water, which is applied in the direction of being fixed on/in an organic carrier, can solve problems such as the large influence of enzyme activity, and achieve the effect of ensuring biological activity.

Inactive Publication Date: 2017-10-20
EN科技有限公司
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Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for preparing enzyme hydrogel particles under mild conditions, aiming to solve the problem of using high pressure, high temperature and oil solvent in the existing methods of encapsulating enzymes. Conditions such as those that have a greater impact on enzyme activity

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  • Enzyme hydrogel particle and preparation method thereof
  • Enzyme hydrogel particle and preparation method thereof
  • Enzyme hydrogel particle and preparation method thereof

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Embodiment Construction

[0028] The present invention provides an enzyme hydrogel particle and a preparation method thereof. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0029] see figure 1 , figure 1 It is a flow chart of a preferred embodiment of the preparation method of a kind of enzyme hydrogel particle of the present invention, as shown in the figure, it comprises steps:

[0030] S100, passing the enzyme aqueous solution and the sodium alginate solution into the inner and outer pipes of the metal microfluidic nozzle respectively;

[0031] Specifically, the aqueous enzyme solution to be wrapped and the sodium alginate solution used to wrap the enzyme are prepared in advance, wherein the concentration of the aqueous enzym...

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Abstract

The invention discloses an enzyme hydrogel particle and a preparation method thereof, wherein the method comprises the following steps of A, introducing an enzyme aqueous solution and a sodium alginate solution into inner-layer and outer-layer pipelines in a metallic microfluid spray head respectively; B, controlling the flow rates of the enzyme aqueous solution and the sodium alginate solution, making the enzyme aqueous solution and the sodium alginate solution mutually contact and form a biphase emulsion liquid drop which is of a core-shell structure; C, adding the biphase emulsion liquid drop into a calcium chloride aqueous solution, so that the enzyme hydrogel particle is made after a shell layer in the biphase emulsion liquid drop is cross-linked and solidified. By adopting the preparation method provided by the invention, the influences of the changes of ambient pressure, potential of hydrogen and a temperature on an enzyme can be effectively avoided; the biological activity of the enzyme is effectively guaranteed.

Description

technical field [0001] The invention relates to the field of enzyme preservation, in particular to an enzyme hydrogel particle and a preparation method thereof. Background technique [0002] Enzyme, also known as enzyme, refers to a polymer substance with biocatalytic function. Enzymes can speed up the speed of chemical reactions (that is, they have a catalytic effect), and the mechanism is to speed up the speed of chemical reactions by reducing the activation energy of the reaction. Some enzymes can convert reactants into products millions of times faster. The activity and application of each enzyme comes from the active site and its specificity of each enzyme, and the active site is a three-dimensional protein structure, its activity and enzyme kinetics are easily affected by environmental factors such as temperature, pH value, etc., such as temperature When the temperature and pH value are beyond the optimum, the activity of the enzyme will decrease significantly. [0...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04
CPCC12N11/10C12N11/04
Inventor 刘洲岑浩璋何颖芝
Owner EN科技有限公司
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