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A kind of method for distinguishing natural curcumin and synthetic curcumin

A technology of curcumin and total curcumin, which is applied in the field of natural pigment identification, can solve the problems of unseen natural curcumin and synthetic curcumin methods, and achieve rapid results

Active Publication Date: 2019-09-17
HENAN ZHONGDA HENGYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the method of identifying natural curcumin and synthetic curcumin

Method used

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  • A kind of method for distinguishing natural curcumin and synthetic curcumin
  • A kind of method for distinguishing natural curcumin and synthetic curcumin
  • A kind of method for distinguishing natural curcumin and synthetic curcumin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: aromatic curcumone standard curve

[0039] With methanol as solvent, standard solutions of aromatic curcumone series with concentrations of 0, 0.05, 0.5, 1, 5, and 10 mg / L were prepared, detected by gas chromatography-tandem mass spectrometer, and quantified by external standard method. The specific operation is as follows:

[0040] Chromatographic column: HP-5MS, 30m×0.25mm×0.25μm, or equivalent;

[0041] Column temperature: keep at 80°C for 2 minutes, rise to 280°C at a rate of 15°C / min, hold for 0 min, rise to 300°C at a rate of 20°C / min, and hold for 2 minutes;

[0042] Post run: 300°C, 3min;

[0043] Injection port temperature: 250°C;

[0044] Transfer line temperature: 250°C;

[0045] Carrier gas: helium, purity ≥99.999%

[0046] Carrier gas flow rate: 1mL / min;

[0047] Injection volume: 1μL;

[0048] Injection method: split injection, split ratio: 20:1;

[0049] Ion source: electron bombardment ion source (EI source);

[0050] Ion source te...

Embodiment 2

[0060] Embodiment 2: the identification of natural curcumin and synthetic curcumin

[0061] Select 10 samples of curcumin from different sources in the market, and follow the steps below:

[0062] 1) Weigh about 0.5g of the sample to be tested, dissolve it with methanol and set the volume to a 50mL volumetric flask, shake well, ultrasonicate for 3min, dilute 10 times with methanol after fully dissolved, and filter with a 0.22 μm organic microporous membrane to obtain liquid to be tested;

[0063] 2) Utilize national standard detection method to measure liquid to be tested, determine the content of total curcumin in the test sample;

[0064] 3) Weigh about 0.5g of the sample to be tested, dissolve it with methanol and set the volume to a 50mL volumetric flask, shake well, ultrasonicate for 3 minutes, dilute 10 times with methanol after fully dissolved, and filter with a 0.22 μm organic microporous membrane to obtain liquid to be tested;

[0065] 4) Utilize gas chromatography...

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Abstract

The invention discloses a method for identification of natural curcumin and synthetic curcumin. The method includes: firstly taking methanol as the solvent to prepare ar-turmerone series standard solutions of different concentrations, using a gas chromatography-tandom mass spectrometer to determine ar-turmerone so as to obtain a multi-reaction monitoring chromatogram, and adopting the ar-turmerone concentration as the abscissa, taking the peak area as the ordinate to draw a standard curve of the peak area and concentration, and using the obtained multi-reaction monitoring chromatogram and standard curve as the reference standard; and then employing the gas chromatography-tandom mass spectrometer to determine a to-be-determined substance by the same method, comparing the measurement result with the reference standard, conducting qualitative analysis according to the retention time of ar-turmerone and qualitative / quantitative ion abundance ratio, and performing quantitative analysis according to the peak area and standard curve of the to-be-determined substance. If the ratio of the ar-turmerone content to the total curcumin content in the to-be-determined substance is 0.5 per thousand or above, the to-be-determined substance is natural curcumin, otherwise the to-be-determined substance is synthetic curcumin. The method provided by the invention is fast, efficient and accurate.

Description

technical field [0001] The invention belongs to the technical field of natural pigment identification, and in particular relates to a method for identifying natural curcumin and synthetic curcumin. Background technique [0002] Curcumin is a polyphenolic substance with a relatively small molecular weight, mainly including curcumin, demethoxycurcumin, bisdemethoxycurcumin and other components. Curcumin is an excellent food colorant and is widely used as a food additive. Because of its antioxidant, anti-inflammatory, anti-cancer and cardiovascular disease prevention and many other pharmacological effects, curcumin is widely used in cosmetics, medicine and other fields. It also has a wide range of applications. [0003] At present, the production methods of curcumin include natural extraction and chemical synthesis. The chemical synthesis method uses vanillin and acetylacetone as raw materials, and is prepared by chemical synthesis; the natural extraction method is naturally ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88
Inventor 韩海涛金子恒李轩焦军伟马国徽宋超宋培培万建锋
Owner HENAN ZHONGDA HENGYUAN BIOTECH CO LTD
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