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Kit and system for predicting neoadjuvant chemotherapeutic effect of ER(+) breast cancer

A technology for breast cancer and kits, applied in measuring devices, color/spectral characteristic measurements, instruments, etc., can solve the problems of less discussion on the correlation between IHC4score and pCR, and achieve a wide range of applications, low cost, and high prediction accuracy Effect

Inactive Publication Date: 2017-10-20
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, few papers have explored the correlation between IHC4score and pCR in the literature

Method used

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  • Kit and system for predicting neoadjuvant chemotherapeutic effect of ER(+) breast cancer
  • Kit and system for predicting neoadjuvant chemotherapeutic effect of ER(+) breast cancer
  • Kit and system for predicting neoadjuvant chemotherapeutic effect of ER(+) breast cancer

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Embodiment 1

[0024] 1. Experimental method

[0025] (A) IHC detection of the expression of the target protein in breast cancer tissue

[0026] Slicing: take the paraffin-embedded specimens fixed in formalin solution, slice them continuously on a tissue slicer, the thickness of the slices is about 4 μm, mount the slices, bake them in a constant temperature oven at 60°C overnight, and dry them for later use;

[0027] Baking slices: paraffin sections are placed at 45°C for 2h or at 60°C for 1h;

[0028] Dewaxing: soak the slices in 2 cylinders of fresh xylene for dewaxing, soaking each cylinder for 15 minutes;

[0029] Gradient ethanol hydration: gradient immersion of slices, followed by 100% ethanol, 95% ethanol, 60% ethanol, ddH 2 In O, the soaking process is about 1-2min in total or 1-2min each to fully hydrate;

[0030] Inactivation of endogenous peroxidase: Shake the glass slide dry, add endogenous peroxidase blocking agent (3% hydrogen peroxide) dropwise, the amount of 3% hydrogen pe...

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Abstract

The invention provides a kit and a system for predicting a neoadjuvant chemotherapeutic effect of ER(+) breast cancer. A new predicting system is established by combining prognosis related conventional clinic pathological factors with immunohistochemical molecular markers to quickly and conveniently provide evidence of predicting clinical chemotherapeutic effect and prognosis with low cost, so that the demand of most of tumor patients can be met. The kit and system are extremely wide in application range, and the predicting accuracy is high through clinical verification.

Description

technical field [0001] The invention relates to a kit and a system for predicting the effect of neoadjuvant chemotherapy for ER (+) breast cancer. Background technique [0002] Breast cancer is the most common malignant tumor in women, and there are about 2 million new breast cancer patients worldwide every year. Breast cancer is a type of highly heterogeneous tumor at the molecular level. There are great differences in histomorphology, immunophenotype, biological behavior and treatment response. Patients with the same traditional pathological TNM staging have great influence on clinical treatment. Response and prognosis can vary widely. [0003] ER(+) breast cancer is the most common molecular subtype of breast cancer, with an incidence rate of about 65% to 70% in the population. The prognosis is the best in breast cancer, and there are more patients in the early stage. Compared with other molecular subtypes of breast cancer, ER(+) breast cancer has a better prognosis, b...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N21/29
CPCG01N21/29G01N33/57415G01N2800/52
Inventor 龚畅宋尔卫谭维格
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV