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A soil micro-ecological environment restoration method for eliminating continuous cropping obstacles

A technology for environmental remediation and continuous cropping obstacles, applied in the field of soil remediation, can solve the problems of unbalanced microbial population ratio, uneven distribution of soil nutrients, and ineffective decomposition of fertilizers, etc., to achieve the effect of preventing diseases and eliminating continuous cropping obstacles

Active Publication Date: 2018-03-16
上海乾界生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In continuous cropping soil, due to the cultivation of the same crop year after year, a large number of harmful microbial populations are bred in the soil, while the activities of beneficial microorganisms such as nitrifying bacteria and ammonifying bacteria in the soil are inhibited, so that the microbial flora of the soil occurs As a result, the proportion of microorganisms in the soil is unbalanced, and the applied fertilizer cannot be effectively decomposed, resulting in uneven distribution of soil nutrients and exacerbating the spread of soil-borne diseases.
[0005] At present, there are various methods to relieve continuous cropping obstacles, including physical methods and chemical methods, such as application of microbial agents, high-temperature sterilization, increasing application of organic fertilizers, rational irrigation, grafting and root replacement, guest-soil methods, etc., but only rely on some simple methods It is impossible to prevent and control continuous cropping obstacles, and it is necessary to carry out targeted prevention and treatment according to the causes of continuous cropping obstacles in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The preparation method of Pichia aumeri granule is as follows:

[0055] a), seed liquid preparation

[0056] On a sterile operating table, inoculate the Pichia aumerii strain into the seed medium, and cultivate the bacteria in a shaker at 28°C and 180rpm / min until the concentration of the bacteria reaches 10 8 CFU / mL, to obtain the seed solution;

[0057] b), fermentation culture

[0058] Add 5mL of the above seed liquid into the fermentation medium, cultivate in a shaker at 28°C and 180rpm / min, detect the number of bacteria in the fermentation bacteria, and wait until the number of bacteria reaches 10 10 Stop fermentation when CFU / mL, obtain Pichia aumeri yeast liquid;

[0059] c) air-drying the above-mentioned Pichia aumeri yeast liquid at 25° C. for 48 hours to obtain solid particles of Pichia aumeri yeast.

[0060] The pH of the described seed medium is 7.0, containing the following materials in parts by weight: 2 g of ammonium chloride, 5 g of sodium acetate, 0.1...

Embodiment 2

[0063] The preparation method of Hansenula anomaly granules is as follows:

[0064] a), seed liquid preparation

[0065] On a sterile operating table, inoculate the strain of Hansenula anomalies into the seed medium, and cultivate the bacteria in a shaker at 28°C and 180rpm / min until the concentration of the bacteria reaches 10 8 CFU / mL, to obtain the seed solution;

[0066] b), fermentation culture

[0067] Add 5mL of the above seed liquid into the fermentation medium, cultivate in a shaker at 28°C and 180rpm / min, detect the number of bacteria in the fermentation bacteria, and wait until the number of bacteria reaches 10 10 When the CFU / mL was reached, the fermentation was stopped, and the abnormal Hansenula yeast liquid was obtained;

[0068] c) Air-dry the above-mentioned Hansenula anomalies bacterial liquid at 25° C. for 48 hours to obtain solid particles of Hansenula anomalies.

[0069] The pH of the described seed medium is 7.0, containing the following materials in ...

Embodiment 3

[0072] The preparation method of the yeast granule of S. forticulata is as follows:

[0073] a), seed solution preparation

[0074] On a sterile operating table, inoculate the yeast strain of S. fumigatus in the seed medium, and cultivate the bacteria in a shaker at 28°C and 180rpm / min until the concentration of the bacteria reaches 10 8 CFU / mL, to obtain the seed solution;

[0075] b), fermentation culture

[0076] Add 5mL of the above seed liquid into the fermentation medium, cultivate in a shaker at 28°C and 180rpm / min, detect the number of bacteria in the fermentation bacteria, and wait until the number of bacteria reaches 10 10 When the CFU / mL is reached, the fermentation is stopped to obtain the yeast liquid of S. fumigatus;

[0077] c) air-drying the above yeast liquid of S. forticulata at 25° C. for 48 hours to obtain solid particles of S. forticulata.

[0078] The pH of the described seed medium is 7.0, containing the following materials in parts by weight: 2 g of...

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Abstract

The invention discloses a soil micro-ecological environment restoration method for eliminating a continuous cropping obstacle. The soil micro-ecological environment restoration method for eliminating the continuous cropping obstacle is realized by combining a soil reducing microbial inoculum and a technology of closing a greenhouse at a high temperature after irrigation. Compared with the prior art, the soil micro-ecological environment restoration method for eliminating the continuous cropping obstacle, disclosed by the invention, has the good characteristics of being environment-friendly, sustainable, easy to operate, and convenient to popularize.

Description

technical field [0001] The invention relates to the technical field of soil restoration, in particular to a soil micro-ecological environment restoration method for eliminating continuous cropping obstacles. Background technique [0002] Continuous cropping obstacle refers to the continuous planting of the same or similar plant in the same field, which makes the plant grow slowly, and even the occurrence of diseases leads to a decrease in yield. Japan calls it "the bogey ground phenomenon", and Europe and the United States call it disease replanting or replanting problem. The main causes of continuous cropping obstacles are divided into five categories: first, the lack of soil nutrients; second, the abnormal response of the soil; third, the continuous deterioration of soil physical and chemical properties; Changes in the content of soil microflora. [0003] The continuous planting of a single crop forms a special soil environment. The beneficial microorganisms in the soil ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C05G3/04C05G3/80
CPCB09C1/10B09C1/105B09C2101/00C05B7/00C05G3/80C05D3/02C05F3/00C05F5/002C05F11/00C05F11/08
Inventor 林江一
Owner 上海乾界生物科技有限公司
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