Preparation method of choline glycerophosphate
A technology of glycerol phosphatidyl choline and soybean lecithin is applied in the field of preparation of glycerol phosphatidyl choline, and can solve the problems of complicated preparation method and the like
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[0014] The invention provides a preparation method of glycerol phosphatidylcholine, wherein the preparation method comprises:
[0015] 1) filter after mixing soybean lecithin and methanol, remove filter residue, obtain clear liquid M1;
[0016] 2) adding tetrabutylammonium hydroxide solution to the clear liquid M1 prepared in step 1) and then placing it under the condition that the stirring rate is 30-100r / min, stirring and mixing for 30-100min to obtain mixture M2;
[0017] 3) adding acetone to the mixture M2 prepared in step 2), and removing the acetone layer after mixing to obtain a crude extract M3;
[0018] 4) The crude extract M3 obtained in step 3) is separated and collected by wave layer chromatography to obtain glycerophosphatidylcholine.
[0019] In the present invention, after the soybean phospholipid and methanol are mixed, the residue is filtered, the clear liquid is taken, and then the clear liquid is stirred and mixed with the tetrabutylammonium hydroxide solut...
Embodiment 1
[0031] 1) 10 parts by weight of soybean lecithin and 80 parts by weight of methanol are placed under the condition that the stirring rate is 100r / min, stirring and mixing after the filtration, removing the filter residue, obtaining clear liquid M1;
[0032] 2) adding 5 weight parts of tetrabutylammonium hydroxide solution (concentration is 3 weight %) in the clear liquid M1 obtained in step 1) and placing the temperature in a water bath heating condition of 30° C., and the stirring rate is 30r Under the condition of / min, stir and mix for 30min to obtain mixture M2;
[0033] 3) adding 120 parts by weight of acetone to the mixture M2 prepared in step 2), and removing the acetone layer after mixing to obtain a crude extract M3;
[0034] 4) using the crude extract M3 prepared in step 3) as a developing agent with a mixture of n-propanol and ammonia water (concentration of 10% by weight) (n-propanol and ammonia water weight ratio is 50:50), and using a silica gel plate as a chroma...
Embodiment 2
[0036] 1) 10 parts by weight of soybean lecithin and 120 parts by weight of methanol are placed under the condition that the stirring speed is 500r / min and stirred and mixed and filtered, and the filter residue is removed to obtain clear liquid M1;
[0037] 2) adding 8 parts by weight of tetrabutylammonium hydroxide solution (concentration is 7% by weight) in the clear liquid M1 obtained in step 1) and placing the temperature in a water bath heating condition of 40° C., and the stirring rate is 100r Under the condition of / min, stir and mix for 100min to obtain mixture M2;
[0038] 3) adding 160 parts by weight of acetone to the mixture M2 prepared in step 2), and removing the acetone layer after mixing to obtain a crude extract M3;
[0039] 4) using the crude extract M3 prepared in step 3) as developing agent with the mixture of n-propanol and ammonia water (concentration is 30% by weight) (n-propanol and ammonia water weight ratio is 75:25), and using silica gel plate as chr...
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