Extracting method of bovine pituitary extract

An extraction method and extract technology, applied in the field of biomedicine, can solve the problems of low extraction efficiency, inability to large-scale production, complex extraction method, etc., and achieve the effects of high extraction efficiency, suitable for large-scale production, and saving pretreatment time.
CN107299077AActive Publication Date: 2017-10-27GUANGDONG BOXI BIO TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGDONG BOXI BIO TECH CO LTD
Publication Date
2017-10-27

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Abstract

The invention discloses an extracting method of a bovine pituitary extract. The method comprises the following steps: obtaining a fresh bovine pituitary, removing blood stains and impurities on a tissue surface, removing external adhesive tissue and a white envelope, and exposing the whole pituitary tissue; adding a protease inhibitor into the pituitary tissue, crushing into paste, then adding a protein extracting liquid, performing stirring and centrifuging sequentially at low temperature of 0-4 DEG C, and collecting supernate; and sterilizing the supernate to obtain the bovine pituitary extract. With the adoption of the method, the bovine pituitary extract contains more than 15g / ml of protein; the bovine pituitary extract can be applied to cell culture and tissue engineering culture. The extracting method is simple, high in extracting efficiency, and suitable for mass production, thereby providing a strong foundation for mass production of the bovine pituitary extract.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for extracting bovine pituitary gland extract. Background technique

[0002] As a substitute for bovine serum, bovine pituitary extract (BPE) is an important additive in serum-free medium, which can effectively remove the adverse effects of complex components in serum on cells, especially for stem cells and stem cells. Pro-differentiation and pro-aging effects of cells, so as to ensure the biological characteristics of cells. It is often used in the cultivation of keratinocytes, melanocytes, oral mucosa cells and other epithelial cells, mesenchymal cells and other cells and in the production of tissue engineering products containing the above cells. The main components of its function are ethanolamine, fatty acids and various protein factors and hormones contained in it, such as growth hormone (GH), thyroid stimulating hormone (TSH), adrenocorticotropic ...

Claims

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