Bovine infectious rhinotracheitis virus ibrv-b strain and its application

A rhinotracheitis virus, IBRV-B technology, applied in the direction of viruses, antiviral agents, viruses/phages, etc.

Active Publication Date: 2020-11-03
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no self-developed vaccine in the domestic market for the prevention and treatment of this disease

Method used

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  • Bovine infectious rhinotracheitis virus ibrv-b strain and its application
  • Bovine infectious rhinotracheitis virus ibrv-b strain and its application
  • Bovine infectious rhinotracheitis virus ibrv-b strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and culture identification of bovine infectious rhinotracheitis virus IBRV-B strain

[0024] 1 Source and standard of virus seeds

[0025] According to the new biological product declaration requirement, in conjunction with a large amount of test data that the present invention obtains, with reference to " Chinese Veterinary Pharmacopoeia " (2005 edition), the poisonous species used for making seedlings has been identified, and the poisoned species standard of the trial procedure (draft) is now carried out as follows illustrate.

[0026] 1.1 Source of poisonous seeds

[0027] The virus used to manufacture this product is Bovine Infectious Rhinotracheitis Virus IBRV-B strain, which was obtained by the inventor after self-isolation and domestication from diseased cattle in 2015. Compared with other isolated strains, it has a higher proliferation titer on cells and has better immunogenicity to cattle, so it is selected for the production of vaccines. ...

Embodiment 2

[0054] The preparation of embodiment 2 inactivated vaccines

[0055] The preparation process of the bovine infectious rhinotracheitis virus IBRV-B strain is prepared according to the current mature commercial vaccine preparation process. Judging from the quality inspection results of 3 batches of vaccines prepared in the laboratory and 5 batches of intermediate trial-produced vaccines, the quality of the vaccine products prepared by this process is stable, and the efficacy inspection results fully meet the established quality standards.

[0056] 1 Preparation of virus solution

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Abstract

The invention discloses an IBRV-B (infectious bovine rhinotracheitis virus-B) strain and application thereof. The IBRV-B strain provided by the invention has the advantages of good immunogenicity and good culture characteristics. The IBRV-B strain is inoculated with MDBK cells; a culture material is harvested; beta-propiolactone is used for inactivation; then, the materials are mixed and emulsified with commercial Montanide<TM> ISA15AVG adjuvants of a French SEPPIC company to be made into an IBRV inactivated vaccine. Experiments prove that when the IBRV inactivated vaccine prepared by using the IBRV-B strain provided by the invention is used for immunizing ablactation health negative cow, the diseases caused by the IBRV can be prevented, so that the vaccine has the advantages of high safety, capability of fast antibody generation, long immunity period and the like.

Description

technical field [0001] The invention belongs to the field of biology, more specifically, belongs to the field of biological vaccines, relates to a bovine infectious rhinotracheitis virus and its application, in particular to a bovine infectious rhinotracheitis virus IBRV-B strain isolated by itself and its The inactivated vaccine prepared by this virus strain. Background technique [0002] Infectious bovine rhinotracheitis virus (IBRV) can cause acute, febrile, and contact infectious diseases in cattle, manifested as inflammation of the upper respiratory tract and tracheal mucosa, dyspnea, runny nose and other symptoms, and can also cause purulent vulva Inflammation, vaginitis, balanitis, conjunctivitis, miscarriage, calf meningitis, mastitis, metritis and other symptoms. The disease was first discovered in Colorado in the United States in the early 1950s, and then other states also discovered the disease one after another. In 1956, Madm and others isolated the virus from ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/265A61P31/22C12R1/93
CPCA61K39/12A61K2039/5252C12N7/00C12N2710/16721C12N2710/16734
Inventor 崔尚金赵占中梁琳
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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