Construction and application of inflammation molecule (PSGL-1) missing abnormal lipid metabolism model

A technology of abnormal lipid metabolism and PSGL-1, which can be used in the construction of transgenic animal models and can solve problems such as lack of models

Inactive Publication Date: 2017-10-27
GUANGDONG PHARMA UNIV
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Problems solved by technology

However, there is still a lack of good models to study the re

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  • Construction and application of inflammation molecule (PSGL-1) missing abnormal lipid metabolism model
  • Construction and application of inflammation molecule (PSGL-1) missing abnormal lipid metabolism model
  • Construction and application of inflammation molecule (PSGL-1) missing abnormal lipid metabolism model

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Embodiment Construction

[0015] 1 Experimental principle

[0016] P-selectin glycoprotein ligand-1 ((PSGL-1)) is an adhesion molecule that expresses sialylated and fucosylated glycans discovered in the early 1990s and belongs to type I transmembrane proteins. (PSGL-1) can not only recognize and capture leukocytes from blood during the initial adhesion process, but also act as a signal transduction molecule to transmit signals into cells and activate leukocytes ( figure 1 ). It is now known that (PSGL-1) has a regulatory role in the inflammatory response. The model constructed by the present invention has focused on the (PSGL-1) deletion on LDLR - / - Effects on lipid metabolism and atherosclerotic plaque formation in mice.

[0017] 2 Experimental methods

[0018] 2.1 Hybrid expansion of genetically engineered mice using LDLR - / - and (PSGL-1) - / - Crossbreed to obtain F1 generation (PSGL-1) + / - ;LDLR + / - Mice; After F1 generation mice are crossed, 4 different genotype mice can be obtained, namely:...

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Abstract

The invention discloses construction and application of an inflammation molecule (PSGL-1) missing abnormal lipid metabolism model. A construction method of the model comprises: based on an abnormal lipid metabolism model-LDLR (Low Density Lipoprotein Receptor) knockout mouse, i.e., an LDLR<-/-> mouse, carrying out modification on a PSGL-1 gene in a genome of the LDLR<-/-> mouse to enable expression of a PSGL-1 protein to be blocked so as to obtain a PSGL-1 missing and lipid metabolism disorder combined transgenic animal model-an LDLR<-/->, PSGL-1<-/-> mouse. Research finds that after the mouse is induced for 12 weeks by high fat diet, relative to the LDLR<-/-> mouse, the newly constructed LDLR<-/->, PSGL-1<-/-> mouse has the advantages that contents of total cholesterol and low density lipoprotein in the serum are reduced, and content of high density lipoprotein is increased; the LDLR<-/->, PSGL-1<-/-> mouse has the advantages that hepatic lobule fatty degeneration is obviously reduced, and the areas of atherosclerotic plaques of aortas and aorta roots are obviously reduced. The model provides a new research carrier for systematically researching a mutual relationship of inflammation molecule and lipid metabolism and searching new drug target and treatment methods.

Description

technical field [0001] The present invention relates to a construction method and application of a transgenic animal model, in particular to a construction method and application of a novel transgenic animal model lacking inflammatory molecule (PSGL-1) combined with abnormal lipid metabolism. Background technique [0002] With the change of people's diet and living habits, the incidence of metabolic diseases is increasing year by year. Abnormal lipid metabolism can lead to diseases such as hypertension and atherosclerosis, which seriously threaten human health. Studies have shown that inflammatory molecules are closely related to the occurrence and development of abnormal lipid metabolism, and inflammation runs through the entire process of disease occurrence and development. However, the current research on inflammation and lipid metabolism is only limited to the level of cells and clinical tissues, and lacks dynamic overall research. A mouse tool model of inflammatory mol...

Claims

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Application Information

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IPC IPC(8): C12N15/87A01K67/027
CPCA01K67/0275A01K2227/105A01K2267/0306C12N15/87
Inventor 杨永霞王丽京黎冰林李江超何晓东王佳
Owner GUANGDONG PHARMA UNIV
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