Amino acid fermentation method using corn steep liquor hydrolysate instead of industrial phosphate

A corn steep liquor hydrolyzate and fermentation method technology, applied in the field of biological fermentation engineering, can solve the problems of waste, loss of phosphorus elements, etc., achieve the effects of simple operation, lower comprehensive production cost, and provide enterprise economic benefits

Inactive Publication Date: 2017-10-27
LINGHUA GROUP INCORPORATED COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the soaking process of corn, most of the phytic acid is dissolved, but most microorganisms lack enzymes that can decompose phytic acid, and the phosphorus in phytic acid is difficult to be used, resulting in the loss and waste of phosphorus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The amino acid fermentation method uses corn steep liquor hydrolyzate instead of industrial phosphate, and uses corn steep steep liquor hydrolyzate hydrolyzed by phytase to replace corn steep liquor and phosphoric acid for feeding in the fermentation process. Specific steps are as follows:

[0024] (1) Take 50g of corn steep liquor, adjust the pH to 4.0, add 0.025g of phytase 1, and stir evenly;

[0025] (2) Hydrolyze at 50°C for 6 hours to obtain corn steep liquor hydrolyzate;

[0026] (3) The hydrolyzate obtained in step (2) is used for fermentation to produce glutamic acid.

[0027] Among them, phytase 1 is phytase 340, with an enzyme activity of 30000u, which was purchased from Weifang Kangdien Biotechnology Co., Ltd.

Embodiment 2

[0029] The amino acid fermentation method uses corn steep liquor hydrolyzate instead of industrial phosphate, and uses corn steep steep liquor hydrolyzate hydrolyzed by phytase to replace corn steep liquor and phosphoric acid for feeding in the fermentation process.

[0030] (1) Take 50g of corn steep liquor, adjust the pH to 5.0, add 0.04g of phytase 2, and stir evenly;

[0031] (2) Hydrolyze at 65°C for 9 hours to obtain corn steep liquor hydrolyzate;

[0032] (3) The hydrolyzate obtained in step (2) is used for fermentation to produce threonine.

[0033] Among them, phytase 2 is thiram, with an enzyme activity of 5000u, purchased from Weifang Kangdien Biotechnology Co., Ltd.

Embodiment 3

[0035] The amino acid fermentation method uses corn steep liquor hydrolyzate instead of industrial phosphate, and uses corn steep steep liquor hydrolyzate hydrolyzed by phytase to replace corn steep liquor and phosphoric acid for feeding in the fermentation process.

[0036] (1) Take 50g of corn steep liquor, adjust the pH to 4.0, add 0.025g of phytase 1, and stir evenly;

[0037] (2) Hydrolyze at 50°C for 6 hours to obtain corn steep liquor hydrolyzate;

[0038] (3) The hydrolyzate obtained in step (2) is used for fermentation to produce lysine.

[0039] Among them, phytase 1 is phytase 340, with an enzyme activity of 30000u, which was purchased from Weifang Kangdien Biotechnology Co., Ltd.

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Abstract

The invention relates to an amino acid fermentation method using corn steep liquor hydrolysate instead of industrial phosphate, using phytase to hydrolyze corn steep liquor to produce the corn steep liquor hydrolysate with free phosphorus content meeting production needs instead of direct use of phosphorus sources such as the corn steep liquor and phosphoric acid to an amino acid fermentation process. The invention provides a method using the corn steep liquor as a raw material and using the corn steep liquor hydrolysate hydrolyzed by the phytase instead of directly using corn steep liquor and adding phosphoric acid as the phosphorus sources in a normal amino acid fermentation production process, a production indicator is basically consistent with the normal fermentation, the cost of all phytase is far lower than the cost of the phosphoric acid capable of achieving the same effect. Therefore, the method has low cost, can effectively reduce the comprehensive production cost of the amino acid while ensuring normal production, directly provides the enterprise economic benefits, and is suitable for comprehensive promotion in production.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to an amino acid fermentation method using corn steep liquor hydrolyzate instead of industrial phosphate. Background technique [0002] Generally, amino acid fermentation requires carbon sources, nitrogen sources, phosphorus sources, and other trace elements and growth factors. Among them, phosphorus is an essential component of nucleic acid and protein, and an important component of cell membranes (phospholipids). Phosphorus plays an important role in cells. In order to maintain the growth of microorganisms, phosphorus is often added to the medium in the form of phosphate, and the phosphate commonly used in industrial production is K 3 PO 4 ·3H 2 O, K 3 PO 4 、Na 2 HPO 4 12H 2 O, NaH 2 PO 4 2H 2 O et al. [0003] Corn steep liquor is produced by soaking corn in the process of manufacturing starch by wet process and concentrated und...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04
CPCC12P13/04
Inventor 杨玉岭岳希金满德恩王成秋郭脉海高永仇南南殷慧慧
Owner LINGHUA GROUP INCORPORATED COMPANY
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